Abstract

This project will investigate the effects of HIV gp120 on the production of new cells by the thymus. It is known that the receptor for HIV gp120, CD4, is involved in thymocyte maturation, particularly in the maturation of those cells which are the precursors for CD4+ mature peripheral T cells. The exact function of CD4 in thymocyte development is not, however, understood. In this project the effects of HIVgp120, or anti-CD4 on the development of thymocytes in human CD4 transgenic mice, in cultured thymuses from these mice, or in cultured human thymuses will be studied. Signal transduction in different subpopulations of thymocytes initiated by the interaction of gp120 or anti-CD4 with CD4 will also be measured. The results of these experiments will indicate whether or not HIV infection, and production of gp120, will inhibit production of mature T cells by the thymus. The results may also shed some light on the role played by CD4 in thymocyte maturation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI029903-02
Application #
3803992
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
National Jewish Health
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Cotton, M F; Cassella, C; Rapaport, E L et al. (1996) Apoptosis in HIV-1 Infection. Behring Inst Mitt :220-31
Dreyfus, D H; Kelleher, C A; Jones, J F et al. (1996) Epstein-Barr virus infection of T cells: implications for altered T-lymphocyte activation, repertoire development and autoimmunity. Immunol Rev 152:89-110
Takase, K; Kelleher, C A; Terada, N et al. (1996) Dissociation of EBV genome replication and host cell proliferation in anti-IgG-stimulated Akata cells. Clin Immunol Immunopathol 81:168-74
Kelleher, C A; Wilkinson, D A; Freeman, J D et al. (1996) Expression of novel-transposon-containing mRNAs in human T cells. J Gen Virol 77 ( Pt 5):1101-10
Drevets, D A; Elliott, A M (1995) Fluorescence labeling of bacteria for studies of intracellular pathogenesis. J Immunol Methods 187:69-79
Lucas, J J; Szepesi, A; Domenico, J et al. (1995) Effects of iron-depletion on cell cycle progression in normal human T lymphocytes: selective inhibition of the appearance of the cyclin A-associated component of the p33cdk2 kinase. Blood 86:2268-80
Campbell, K S; Bedzyk, W D; Cambier, J C (1995) Manipulation of B cell antigen receptor tyrosine phosphorylation using aluminum fluoride and sodium orthovanadate. Mol Immunol 32:1283-94
Rozdzial, M M; Malissen, B; Finkel, T H (1995) Tyrosine-phosphorylated T cell receptor zeta chain associates with the actin cytoskeleton upon activation of mature T lymphocytes. Immunity 3:623-33
Finkel, T H; Tudor-Williams, G; Banda, N K et al. (1995) Apoptosis occurs predominantly in bystander cells and not in productively infected cells of HIV- and SIV-infected lymph nodes. Nat Med 1:129-34
Lucas, J J; Szepesi, A; Modiano, J F et al. (1995) Regulation of synthesis and activity of the PLSTIRE protein (cyclin-dependent kinase 6 (cdk6)), a major cyclin D-associated cdk4 homologue in normal human T lymphocytes. J Immunol 154:6275-84

Showing the most recent 10 out of 38 publications