Abstract

Four investigators from one clinical and two basic science departments will explore aspects of autoantibody formation and regulation in autoimmune disease and of cell mediated immunity. The first two projects focus on anti-DNA antibodies in systemic lupus, the structural basis for antigen binding and pathogenicity and the regulation of expression DNA binding antibodies. The third project is an exploits an idiotypic approach to understand the molecular genetics of rheumatoid factors in rheumatoid arthritis. The fourth project exploits a novel methodology for studying self peptides associated with MHC antigens to identify tissue specific self peptides. The investigators will share 3 core facilities funded by this grant, an administrative core, an oligonucleotide synthesis core, and an animal core, as well as additional core facilities supported by other sources. The investigators in this program are highly interactive. This application reflects the continuing commitment of some of the investigators to the study of human disease and the new focus of others on issues relevant to human pathobiology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI033184-03
Application #
2068179
Study Section
Special Emphasis Panel (SRC (20))
Project Start
1992-08-15
Project End
1996-04-30
Budget Start
1994-05-01
Budget End
1995-04-30
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Valadon, P; Nussbaum, G; Oh, J et al. (1998) Aspects of antigen mimicry revealed by immunization with a peptide mimetic of Cryptococcus neoformans polysaccharide. J Immunol 161:1829-36
Nussbaum, G; Cleare, W; Casadevall, A et al. (1997) Epitope location in the Cryptococcus neoformans capsule is a determinant of antibody efficacy. J Exp Med 185:685-94
Gaynor, B; Putterman, C; Valadon, P et al. (1997) Peptide inhibition of glomerular deposition of an anti-DNA antibody. Proc Natl Acad Sci U S A 94:1955-60
Teller, K; Budhai, L; Zhang, M et al. (1996) HLA-DRB1 and DQB typing of Hispanic American patients with rheumatoid arthritis: the ""shared epitope"" hypothesis may not apply. J Rheumatol 23:1363-8
Valadon, P; Nussbaum, G; Boyd, L F et al. (1996) Peptide libraries define the fine specificity of anti-polysaccharide antibodies to Cryptococcus neoformans. J Mol Biol 261:11-22
Zhu, M; Green, N S; Rabinowitz, J L et al. (1996) Differential V region mutation of two transfected Ig genes and their interaction in cultured B cell lines. EMBO J 15:2738-47
Sakita, I; Horig, H; Sun, R et al. (1996) In vivo CTL immunity can be elicited by in vitro reconstituted MHC/peptide complex. J Immunol Methods 192:105-15
Zhang, M; Spey, D; Ackerman, S et al. (1996) Rheumatoid factor idiotypic and antigenic specificity is strongly influenced by the light chain VJ junction. J Immunol 156:3570-5
Valadon, P; Scharff, M D (1996) Enhancement of ELISAs for screening peptides in epitope phage display libraries. J Immunol Methods 197:171-9
Sun, R; Shepherd, S E; Geier, S S et al. (1995) Evidence that the antigen receptors of cytotoxic T lymphocytes interact with a common recognition pattern on the H-2Kb molecule. Immunity 3:573-82

Showing the most recent 10 out of 18 publications