The Flow Cytometry Core will meet the special needs of the Program Project members. Theavailability of quality sorting and analysis capabilities is essential for the success of the researchefforts proposed in this Program Project Grant. We will be active users of both the MoFlo cellsorting and LSR II fluorescence analyzer in the studies dealing with: sorting of small numbers ofcells based on either cell surface or transduced gene markers; and multiparameter analyses todetect biochemical and biological changes in rare populations of lymphocytes as well as wholepopulations transduced with various genes. In many cases, the individual program directors willneed access to machines that will require triple or even quadruple staining for the sophisticatedexperiments outlined in the proposal.
The specific aims of this core are 1. To provide high speedcell sorting to isolate cells that can be used for functional and biochemical studies; 2. To provideanalytical flow cytometry using a state-of-the-art LSR II to perform multiparameter analyses and3. To provide innovative approaches to study immune tolerance using flow microscopy. Theavailability of the core ensures that the PPG investigators will have access to this essentialfacility on the UCSF campus.
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