Abstract

The additional research conducted as part of this program will exceed the capacity of the existing Flow Cytometry Facility in the Immunobiology Section at Yale University. This core facility is intended to increase the efficiency of use of the two existing flow cytometers. A FACS operator will be employed to: 1. Become trained in the use of the FACStar Plus cell sorter, and extend the hours during which this machine is usable by working on a 'flexitime' basis, co-ordinated with the schedule of the principal FACStar Plus operator. 2. To allow use of the FACScan analytical flow cytometer for untrained personnel. At present this instrument is restricted to trained individuals, and is therefore unavailable to occasional users or to new members of the Program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI036529-04
Application #
6235267
Study Section
Project Start
1997-06-01
Project End
1998-09-29
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Doyle, Hester A; Mamula, Mark J (2012) Autoantigenesis: the evolution of protein modifications in autoimmune disease. Curr Opin Immunol 24:112-8
Kamanaka, Masahito; Huber, Samuel; Zenewicz, Lauren A et al. (2011) Memory/effector (CD45RB(lo)) CD4 T cells are controlled directly by IL-10 and cause IL-22-dependent intestinal pathology. J Exp Med 208:1027-40
Sweet, Rebecca A; Christensen, Sean R; Harris, Michelle L et al. (2010) A new site-directed transgenic rheumatoid factor mouse model demonstrates extrafollicular class switch and plasmablast formation. Autoimmunity 43:607-18
Choi, Je-Min; Shin, Jae-Hun; Sohn, Myung-Hyun et al. (2010) Cell-permeable Foxp3 protein alleviates autoimmune disease associated with inflammatory bowel disease and allergic airway inflammation. Proc Natl Acad Sci U S A 107:18575-80
Sanjabi, Shomyseh; Zenewicz, Lauren A; Kamanaka, Masahito et al. (2009) Anti-inflammatory and pro-inflammatory roles of TGF-beta, IL-10, and IL-22 in immunity and autoimmunity. Curr Opin Pharmacol 9:447-53
Herlands, Robin A; Christensen, Sean R; Sweet, Rebecca A et al. (2008) T cell-independent and toll-like receptor-dependent antigen-driven activation of autoreactive B cells. Immunity 29:249-60
Shlomchik, Mark J (2008) Sites and stages of autoreactive B cell activation and regulation. Immunity 28:18-28
Christensen, Sean R; Shlomchik, Mark J (2007) Regulation of lupus-related autoantibody production and clinical disease by Toll-like receptors. Semin Immunol 19:11-23
Herlands, Robin A; William, Jacqueline; Hershberg, Uri et al. (2007) Anti-chromatin antibodies drive in vivo antigen-specific activation and somatic hypermutation of rheumatoid factor B cells at extrafollicular sites. Eur J Immunol 37:3339-51
Harvey, Bohdan P; Gee, Renelle J; Haberman, Ann M et al. (2007) Antigen presentation and transfer between B cells and macrophages. Eur J Immunol 37:1739-51

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