Core is responsible for preparing the various peptides. Protegrins will generally be synthesized by Dr. Waring at the UCLA site. LL-37 and related alpha-helical peptides will be synthesized by Dr. Pohl at Emory, as will any protegrins that would be benefit from t-boc synthesis. The availability of high-quality peptides is absolutely essential for the proposed studies. Dr. Waring and Pohl are highly accomplished investigators with access to state-of-the-art tools for peptide synthesis, purification and characterization.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI037945-08
Application #
6654525
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2002-09-01
Project End
2003-07-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Gryllos, Ioannis; Tran-Winkler, Hien J; Cheng, Ming-Fang et al. (2008) Induction of group A Streptococcus virulence by a human antimicrobial peptide. Proc Natl Acad Sci U S A 105:16755-60
Doherty, Tim; Waring, Alan J; Hong, Mei (2008) Dynamic structure of disulfide-removed linear analogs of tachyplesin-I in the lipid bilayer from solid-state NMR. Biochemistry 47:1105-16
Tang, Ming; Waring, Alan J; Hong, Mei (2007) Phosphate-mediated arginine insertion into lipid membranes and pore formation by a cationic membrane peptide from solid-state NMR. J Am Chem Soc 129:11438-46
Mani, Rajeswari; Waring, Alan J; Hong, Mei (2007) Conformation, dynamics, and insertion of a noncysteine-containing protegrin-1 analogue in lipid membranes from solid-state NMR spectroscopy. Chembiochem 8:1877-84
Cole, Amy L; Yang, Otto O; Warren, Andrew D et al. (2006) HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41. J Immunol 176:6900-5
Doherty, Tim; Waring, Alan J; Hong, M (2006) Membrane-bound conformation and topology of the antimicrobial peptide tachyplesin I by solid-state NMR. Biochemistry 45:13323-30
Mani, Rajeswari; Cady, Sarah D; Tang, Ming et al. (2006) Membrane-dependent oligomeric structure and pore formation of a beta-hairpin antimicrobial peptide in lipid bilayers from solid-state NMR. Proc Natl Acad Sci U S A 103:16242-7
Valore, Erika V; Wiley, Dorothy J; Ganz, Tomas (2006) Reversible deficiency of antimicrobial polypeptides in bacterial vaginosis. Infect Immun 74:5693-702
Doherty, Timothy; Waring, Alan J; Hong, Mei (2006) Peptide-lipid interactions of the beta-hairpin antimicrobial peptide tachyplesin and its linear derivatives from solid-state NMR. Biochim Biophys Acta 1758:1285-91
Mani, R; Tang, M; Wu, X et al. (2006) Membrane-bound dimer structure of a beta-hairpin antimicrobial peptide from rotational-echo double-resonance solid-state NMR. Biochemistry 45:8341-9

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