Abstract

The central unifying theme of this Project is to define the structure and function of pattern recognition receptors in Drosophila and man. We build on our past expertise in the Laboratory of Developmental Immunology where we have developed an approach that integrates biochemistry, cell biology, genetics, proteomics, and structural biology to identify and characterize biologically relevant pattern recognition molecules. We plan to build on and extend our previous work that has benefited from the collaboration between the four laboratories and an administrative core of the PPG. We have three aims: (1) explore the structure and function of a novel pattern recognition receptor Eater (in collaboration with Projects 2, 3 and 4); (2) utilize a combination of approaches in Drosophila and man to identify the macrophage receptor for anthrax spores; (3) examine the structure and function of peptidoglycan binding proteins in flies and man (in collaboration with Projects 2, 3 and 4)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI044220-08
Application #
7269430
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2006-07-01
Budget End
2007-06-30
Support Year
8
Fiscal Year
2006
Total Cost
$387,894
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Gendrin, Mathilde; Turlure, Fanny; Rodgers, Faye H et al. (2017) The Peptidoglycan Recognition Proteins PGRPLA and PGRPLB Regulate Anopheles Immunity to Bacteria and Affect Infection by Plasmodium. J Innate Immun 9:333-342
Lombardo, Fabrizio; Christophides, George K (2016) Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection. Parasit Vectors 9:78
Lombardo, Fabrizio; Ghani, Yasmeen; Kafatos, Fotis C et al. (2013) Comprehensive genetic dissection of the hemocyte immune response in the malaria mosquito Anopheles gambiae. PLoS Pathog 9:e1003145
Cezairliyan, Brent; Vinayavekhin, Nawaporn; Grenfell-Lee, Daniel et al. (2013) Identification of Pseudomonas aeruginosa phenazines that kill Caenorhabditis elegans. PLoS Pathog 9:e1003101
Feinbaum, Rhonda L; Urbach, Jonathan M; Liberati, Nicole T et al. (2012) Genome-wide identification of Pseudomonas aeruginosa virulence-related genes using a Caenorhabditis elegans infection model. PLoS Pathog 8:e1002813
Chung, Yoon-Suk Alexander; Kocks, Christine (2012) Phagocytosis of bacterial pathogens. Fly (Austin) 6:21-5
Whiteman, Noah K; Gloss, Andrew D; Sackton, Timothy B et al. (2012) Genes involved in the evolution of herbivory by a leaf-mining, Drosophilid fly. Genome Biol Evol 4:900-16
Pukkila-Worley, Read; Ausubel, Frederick M (2012) Immune defense mechanisms in the Caenorhabditis elegans intestinal epithelium. Curr Opin Immunol 24:3-9
Chung, Yoon-Suk Alexander; Kocks, Christine (2011) Recognition of pathogenic microbes by the Drosophila phagocytic pattern recognition receptor Eater. J Biol Chem 286:26524-32
Limmer, Stefanie; Haller, Samantha; Drenkard, Eliana et al. (2011) Pseudomonas aeruginosa RhlR is required to neutralize the cellular immune response in a Drosophila melanogaster oral infection model. Proc Natl Acad Sci U S A 108:17378-83

Showing the most recent 10 out of 88 publications