Abstract

The broad aim of this Project Proposal is to develop the tools that can form the basis of a genetic control strategy for malaria in sub-Saharan African transmitted by the vector Anopheles gambiae. This TDRU Program Project involves 4 specific projects.
The specific aims of Project 1 are the development of two different techniques for germ lines transformation of An. Gambiae, a transposon-based method and a site- specific recombinase method for introducing larger fragments of DNA into the genome.
The aim of project 2 is to develop transgene constructs that can block transmission blocking antibody and another based on the small, antiparasitic peptides like cecropins and megainins.
The aim of project 3 is to develop and test antiparasite transgene constructs based on An. Gambiae immune peptides that will be delivered to the hemocoel. The goal of Project 4 is to test the hypothesis that a meiotic drive system can be used as the necessary tool for driving a genetically engineered parasite refractory genotype (such as those produced in Projects 2 and 3) into the wild population.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI045123-03
Application #
6362408
Study Section
Special Emphasis Panel (ZAI1-VSG-M (J1))
Program Officer
Aultman, Kathryn S
Project Start
1999-03-01
Project End
2004-02-29
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
3
Fiscal Year
2001
Total Cost
$670,167
Indirect Cost

  Institution

Name
University of Notre Dame
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
824910376
City
Notre Dame
State
IN
Country
United States
Zip Code
46556

  Publications

Shin, Dongyoung; Mori, Akio; Severson, David W (2012) Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti. J Hered 103:303-7
Shin, Dongyoung; Jin, Lizhong; Lobo, Neil F et al. (2011) Transcript profiling of the meiotic drive phenotype in testis of Aedes aegypti using suppressive subtractive hybridization. J Insect Physiol 57:1220-6
Chadee, Dave D; Doon, Rohit; Severson, David W (2007) Surveillance of dengue fever cases using a novel Aedes aegypti population sampling method in Trinidad, West Indies: the cardinal points approach. Acta Trop 104:1-7
Cha, Sung-Jae; Mori, Akio; Chadee, Dave D et al. (2006) Cage trials using an endogenous meiotic drive gene in the mosquito Aedes aegypti to promote population replacement. Am J Trop Med Hyg 74:62-8
Cha, Sung-Jae; Lobo, Neil; Debruyn, Becky et al. (2006) Isolation and characterization of the RanGAP gene in the mosquito Aedes aegypti. DNA Seq 17:223-30
Cha, Sung-Jae; Chadee, Dave D; Severson, David W (2006) Population dynamics of an endogenous meiotic drive system in Aedes aegypti in Trinidad. Am J Trop Med Hyg 75:70-7
Kayondo, Jonathan K; Mukwaya, Louis G; Stump, Aram et al. (2005) Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda. Malar J 4:59
Mori, Akio; Chadee, Dave D; Graham, Douglas H et al. (2004) Reinvestigation of an endogenous meiotic drive system in the mosquito, Aedes aegypti (Diptera: Culicidae). J Med Entomol 41:1027-33
Shin, Sang Woon; Kokoza, Vladimir A; Raikhel, Alexander S (2003) Transgenesis and reverse genetics of mosquito innate immunity. J Exp Biol 206:3835-43
Colton, Y M; Chadee, D D; Severson, D W (2003) Natural skip oviposition of the mosquito Aedes aegypti indicated by codominant genetic markers. Med Vet Entomol 17:195-204

Showing the most recent 10 out of 20 publications