Abstract

This core facility will provide the high quality flow cytometry and sorting required by all four of the projects outlined in this proposal. The facility has a dedicated operation who will maintain and operate three instruments. An advanced MoFlo will be used for sterile sorting of single cells or bulk populations. It will be configured with two lasers or UV and visible applications, as well as six fluorescence detectors to allow multiparameter resolution and sorting of up to four populations simultaneously. A FACScan and a FACSCalibur will yield analytical flow cytometry data as required for many of the proposed experiments.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI045864-03
Application #
6491032
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104

  Publications

Joachims, Michelle L; Chain, Jennifer L; Hooker, Scott W et al. (2006) Human alpha beta and gamma delta thymocyte development: TCR gene rearrangements, intracellular TCR beta expression, and gamma delta developmental potential--differences between men and mice. J Immunol 176:1543-52
Yokota, Takafumi; Huang, Jiaxue; Tavian, Manuela et al. (2006) Tracing the first waves of lymphopoiesis in mice. Development 133:2041-51
Igarashi, Hideya; Medina, Kay L; Yokota, Takafumi et al. (2005) Early lymphoid progenitors in mouse and man are highly sensitive to glucocorticoids. Int Immunol 17:501-11
Rajaiya, Jaya; Hatfield, Melissa; Nixon, Jamee C et al. (2005) Bruton's tyrosine kinase regulates immunoglobulin promoter activation in association with the transcription factor Bright. Mol Cell Biol 25:2073-84
Chain, J L; Joachims, M L; Hooker, S W et al. (2005) Real-time PCR method for the quantitative analysis of human T-cell receptor gamma and beta gene rearrangements. J Immunol Methods 300:12-23
Yao, Longbiao; Yokota, Takafumi; Xia, Lijun et al. (2005) Bone marrow dysfunction in mice lacking the cytokine receptor gp130 in endothelial cells. Blood 106:4093-101
Kolar, G R; Capra, J D (2004) Immunoglobulin heavy-chain receptor editing is observed in the NOD/SCID model of human B-cell development. Scand J Immunol 60:108-11
Nixon, Jamee C; Rajaiya, Jaya; Webb, Carol F (2004) Mutations in the DNA-binding domain of the transcription factor Bright act as dominant negative proteins and interfere with immunoglobulin transactivation. J Biol Chem 279:52465-72
Nixon, Jamee C; Rajaiya, Jaya B; Ayers, Neil et al. (2004) The transcription factor, Bright, is not expressed in all human B lymphocyte subpopulations. Cell Immunol 228:42-53
Kolar, Grant R; Yokota, Takafumi; Rossi, Maria Isabel D et al. (2004) Human fetal, cord blood, and adult lymphocyte progenitors have similar potential for generating B cells with a diverse immunoglobulin repertoire. Blood 104:2981-7

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