We propose to use this Program Project Grant as an opportunity to explore a diverse set of important biological issues. The topics fit in well with the collaborative theme of this PPG and will allow my laboratory to explore several new, important avenues of structural virology. A major theme of this grant is to use structural biology methods to examine the processes involved in the vector transmission of plant viruses. Using cucumber mosaic virus (CMV) as a model for viruses that are transmitted via aphids without helper proteins, we plan to examine the structural motifs that interact with aphid mouthparts and are required for aphid transmission. Preliminary work has identified surface residues for aphid transmission. We propose to extend the resolution of the current CMV model. This, combined with immunological and cryo-TEM techniques, will allow use to examine aphid-virus interactions. With poty viruses, a helper protein, HC-Pro, is required to be transmitted by aphids. The N-terminal domain of HC-Pro is cysteine rich and is responsible for vector transmission and systemic spread throughout the plant. As a third approach to understanding transmission of plant viruses, we are pursuing structural studies on the carmovirus, cowpea mottle virus (CPMoV). We have determined the low resolution (approximately 23 angstroms) structure of CPMoV, and the capsid organization is very similar to that of another member of the Tombusviridae tomato bushy stunt virus (TBSV). However, unlike TBSV, CPMoV is not sensitive to RNAse- a property thought to be essential for its transmission by beetles. Structural details of this virus should elucidate the reason underlying these differences in RNAse sensitivity. We will initiate crystallographic studies on the non-structural proteins of the flaviviruses. While this work is just beginning, the PPG will facilitate collaborative efforts on these medically important viruses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI045976-02
Application #
6340735
Study Section
Microbiology and Infectious Diseases B Subcommittee (MID)
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2000
Total Cost
$166,157
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Parent, Kristin N; Gilcrease, Eddie B; Casjens, Sherwood R et al. (2012) Structural evolution of the P22-like phages: comparison of Sf6 and P22 procapsid and virion architectures. Virology 427:177-88
Brodin, Jeffrey D; Ambroggio, X I; Tang, Chunyan et al. (2012) Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays. Nat Chem 4:375-82
Kwon, Soonshin; Chen, Zack C Y; Kim, Ji-Hun et al. (2012) Misfit-guided self-organization of anticorrelated Ge quantum dot arrays on Si nanowires. Nano Lett 12:4757-62
Khadka, Sudip; Vangeloff, Abbey D; Zhang, Chaoying et al. (2011) A physical interaction network of dengue virus and human proteins. Mol Cell Proteomics 10:M111.012187
Tang, Jinghua; Lander, Gabriel C; Olia, Adam S et al. (2011) Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22. Structure 19:496-502
Tang, Jinghua; Jose, Joyce; Chipman, Paul et al. (2011) Molecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus. J Mol Biol 414:442-59
Ku, Ti-Hsuan; Chien, Miao-Ping; Thompson, Matthew P et al. (2011) Controlling and switching the morphology of micellar nanoparticles with enzymes. J Am Chem Soc 133:8392-5
Rossmann, Michael G; Battisti, Anthony J; Plevka, Pavel (2011) Future prospects. Adv Protein Chem Struct Biol 82:101-21
Shen, Peter S; Enderlein, Dirk; Nelson, Christian D S et al. (2011) The structure of avian polyomavirus reveals variably sized capsids, non-conserved inter-capsomere interactions, and a possible location of the minor capsid protein VP4. Virology 411:142-52
Yan, Xiaodong; Parent, Kristin N; Goodman, Russell P et al. (2011) Virion structure of baboon reovirus, a fusogenic orthoreovirus that lacks an adhesion fiber. J Virol 85:7483-95

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