Abstract

This Core will facilitate the testing of lead compounds arising from our various enzyme screens in Projects 1-4, firstly, in an established macrophage infection mouse mode and, secondly, in an established aerosol mouse infection model, by our colleague Dr. Ian Orme and staff, under the support of NIH, NIAID, NCDDG-OI Contract AI-45239, """"""""Animal Model Testing of TB Drugs."""""""" The minimal inhibitory concentration (MIC) of promising leads arising from Projects 1-4 will be tested by Dr. Phetsuksiris, supported through Project 1, using a modified microdrop agar proportion test, and by broth culture, and, in the case of drug-resistant strains, by a microplate-colony protocol. Under this Core, we also plan to conduct limited cytotoxicity testing of promising products by a microplate alamar blue assay before submitting for more comprehensive testing to the NIH-sponsored TAACF (Tuberculosis Antimicrobial Acquisition and Coordinating Facility).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI046393-01
Application #
6254623
Study Section
Special Emphasis Panel (ZAI1-VSG-A (S2))
Project Start
1999-09-30
Project End
2003-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Colorado State University-Fort Collins
Department
Type
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523

  Publications

Amin, Anita G; Angala, Shiva K; Chatterjee, Delphi et al. (2009) Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts. Methods Mol Biol 465:187-201
Wang, Wenjian; Dong, Changjiang; McNeil, Michael et al. (2008) The structural basis of chain length control in Rv1086. J Mol Biol 381:129-40
Parish, Tanya; Schaeffer, Merrill; Roberts, Gretta et al. (2005) HemZ is essential for heme biosynthesis in Mycobacterium tuberculosis. Tuberculosis (Edinb) 85:197-204
Schaeffer, M L Merrill L; Carson, J D Jeffrey D; Kallender, Howard et al. (2004) Development of a scintillation proximity assay for the Mycobacterium tuberculosis KasA and KasB enzymes involved in mycolic acid biosynthesis. Tuberculosis (Edinb) 84:353-60
Brennan, P J (2003) Structure, function, and biogenesis of the cell wall of Mycobacterium tuberculosis. Tuberculosis (Edinb) 83:91-7
Ma, Y; Stern, R J; Scherman, M S et al. (2001) Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose. Antimicrob Agents Chemother 45:1407-16
Escuyer, V E; Lety, M A; Torrelles, J B et al. (2001) The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J Biol Chem 276:48854-62