Abstract

The objective of the TCR core is to provide the Project leaders access to TCR spectratyping technology, single stranded conformational polymorphism (SSCP) analysis, subcloning, sequencing and screening of ap-TCR repertoires, and acquisition of TCR repertoire analytical databases. Projects 1, 2 and 3 will be heavily reliant on the identification of T cell clones specific to vaccinia, yellow fever, influenza A and Epstein- Barr viruses among healthy, diseased, or vaccinated individuals over the 5 year study period. The essential aspects of each proposed study are to define the distributions of virus-specific cells among TCR Va- and Vp- families and to analyze the structural nature and evolution of the TCR a- and p-chains. Each Project leader will initiate the technical procedures in his/her own laboratory and give them to the TCR core for further treatments and analyses. The demonstrated expertise of the Core Director in TCR repertoire analyses will ensure a high quality of TCR analyses throughout the Program Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI049320-10
Application #
8085803
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
10
Fiscal Year
2010
Total Cost
$129,228
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Weiss, Eric R; Lamers, Susanna L; Henderson, Jennifer L et al. (2018) Early Epstein-Barr Virus Genomic Diversity and Convergence toward the B95.8 Genome in Primary Infection. J Virol 92:
Song, InYoung; Gil, Anna; Mishra, Rabinarayan et al. (2017) Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope. Nat Struct Mol Biol 24:395-406
Watkin, Levi B; Mishra, Rabinarayan; Gil, Anna et al. (2017) Unique influenza A cross-reactive memory CD8 T-cell receptor repertoire has a potential to protect against EBV seroconversion. J Allergy Clin Immunol 140:1206-1210
Weiss, Eric R; Alter, Galit; Ogembo, Javier Gordon et al. (2017) High Epstein-Barr Virus Load and Genomic Diversity Are Associated with Generation of gp350-Specific Neutralizing Antibodies following Acute Infectious Mononucleosis. J Virol 91:
Aslan, Nuray; Watkin, Levi B; Gil, Anna et al. (2017) Severity of Acute Infectious Mononucleosis Correlates with Cross-Reactive Influenza CD8 T-Cell Receptor Repertoires. MBio 8:
Gil, Anna; Yassai, Maryam B; Naumov, Yuri N et al. (2015) Narrowing of human influenza A virus-specific T cell receptor ? and ? repertoires with increasing age. J Virol 89:4102-16
Gil, Anna; Kenney, Laurie L; Mishra, Rabinarayan et al. (2015) Vaccination and heterologous immunity: educating the immune system. Trans R Soc Trop Med Hyg 109:62-9
Greenough, Thomas C; Straubhaar, Juerg R; Kamga, Larisa et al. (2015) A Gene Expression Signature That Correlates with CD8+ T Cell Expansion in Acute EBV Infection. J Immunol 195:4185-97
Renzette, Nicholas; Somasundaran, Mohan; Brewster, Frank et al. (2014) Epstein-Barr virus latent membrane protein 1 genetic variability in peripheral blood B cells and oropharyngeal fluids. J Virol 88:3744-55
Chen, Alex T; Cornberg, Markus; Gras, Stephanie et al. (2012) Loss of anti-viral immunity by infection with a virus encoding a cross-reactive pathogenic epitope. PLoS Pathog 8:e1002633

Showing the most recent 10 out of 38 publications