The Flow Cytometry Core will provide essential expertise, support and reagents to program project investigators for quantitative and phenotypic analysis of complex lymphocyte and antigen-presenting cell populations. In addition, considerable expertise is available for the design and implementation of high- throughput cell sorting experiments. The Core director Dr. Aguila, currently Associate Director of the Flow Cytometry Facility at UCHC, trained at Stanford in stem cell isolation by flow cytometry and so is well-versed at analysis and cell sorting of rare populations. The Flow Cytometry Facility is also well-equipped to handle the complexity as well as the volume of samples generated in this Program, having 6 analyzers and two high-speed cell sorters with multicolor capabilities. The equipment includes three BD-LSR 1 machines with two able to perform up to 15-parameter analysis and the third a five laser modified machine to allow up to 21 parameter analysis incuding multiple lines for detection of quantum dot-type reagents. Two five laser FACSAria lis are available for high-speed multicolor sorting and analysis. One of these is housed in a Baker hood to allow sorting of potentially infected tissues from mice or humans. In addition to support for facility costs, the Core will also provide technical assistance for processing, cell sorting, and analyzing samples and data. Having a dedicated research assistant for the Program will ensure uniformity of quality and help to increase efficiency and productivity. For these reasons the Flow Cytometry Core continues to be an integral component of each project and is therefore requisite to the success of this Program.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI056172-09
Application #
8728383
Study Section
Special Emphasis Panel (ZAI1-PA-I)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
9
Fiscal Year
2014
Total Cost
$268,792
Indirect Cost
$78,228
Name
University of Connecticut
Department
Type
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030
Benoun, Joseph M; Peres, Newton G; Wang, Nancy et al. (2018) Optimal protection against Salmonella infection requires noncirculating memory. Proc Natl Acad Sci U S A 115:10416-10421
Ménoret, Antoine; Buturla, James A; Xu, Maria M et al. (2018) T cell-directed IL-17 production by lung granular ?? T cells is coordinated by a novel IL-2 and IL-1? circuit. Mucosal Immunol 11:1398-1407
Svedova, Julia; Ménoret, Antoine; Mittal, Payal et al. (2017) Therapeutic blockade of CD54 attenuates pulmonary barrier damage in T cell-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol 313:L177-L191
Shinde, Paurvi; Liu, Wenhai; Ménoret, Antoine et al. (2017) Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production. J Leukoc Biol 102:57-69
Liu, Wenhai; Menoret, Antoine; Vella, Anthony T (2017) Responses to LPS boost effector CD8 T-cell accumulation outside of signals 1 and 2. Cell Mol Immunol 14:254-253
Lee, Seung-Joo; Benoun, Joseph; Sheridan, Brian S et al. (2017) Dual Immunization with SseB/Flagellin Provides Enhanced Protection against Salmonella Infection Mediated by Circulating Memory Cells. J Immunol 199:1353-1361
Romagnoli, P A; Fu, H H; Qiu, Z et al. (2017) Differentiation of distinct long-lived memory CD4 T cells in intestinal tissues after oral Listeria monocytogenes infection. Mucosal Immunol 10:520-530
Pham, Oanh H; O'Donnell, Hope; Al-Shamkhani, Aymen et al. (2017) T cell expression of IL-18R and DR3 is essential for non-cognate stimulation of Th1 cells and optimal clearance of intracellular bacteria. PLoS Pathog 13:e1006566
Risso, Gabriela S; Carabajal, Marianela V; Bruno, Laura A et al. (2017) U-Omp19 fromBrucella abortusIs a Useful Adjuvant for Vaccine Formulations againstSalmonellaInfection in Mice. Front Immunol 8:171
Benoun, Joseph M; Labuda, Jasmine C; McSorley, Stephen J (2016) Collateral Damage: Detrimental Effect of Antibiotics on the Development of Protective Immune Memory. MBio 7:

Showing the most recent 10 out of 87 publications