Abstract

The Core (Core C) includes the Real-Time PCR instrument (Model Mx4000, Strategene) that permitsMultiplex Quantitative analyses. It has the capability of a total of seven fluorescent primers in themultiplex mode so that seven DNA samples and primer pairs can be analyzed at one time.The facility will be directed by Dr. Yufang Shi. All members of the Program Project will use theinstrument without charge for facility use. Other investigators pay an annual user fee of $400 to helpcover the cost of the Service Contract. The director will train members of the Program Project and anyof their staff at periodic training sessions. The facility will cover 25 % of the cost of Dr. Jian-Nian Zhouwho will care for the facility and provide the data (graphs, data) to the investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI057596-05
Application #
7668604
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2008-11-30
Support Year
5
Fiscal Year
2008
Total Cost
$46,066
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Type
DUNS #
617022384
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Krause, Christopher D; Izotova, Lara S; Pestka, Sidney (2013) Analytical use of multi-protein Fluorescence Resonance Energy Transfer to demonstrate membrane-facilitated interactions within cytokine receptor complexes. Cytokine 64:298-309
Zhang, J; Roberts, A I; Liu, C et al. (2013) A novel subset of helper T cells promotes immune responses by secreting GM-CSF. Cell Death Differ 20:1731-41
Krause, Christopher D; Digioia, Gina; Izotova, Lara S et al. (2013) Improving the spectral analysis of Fluorescence Resonance Energy Transfer in live cells: application to interferon receptors and Janus kinases. Cytokine 64:272-85
Krause, Christopher D; Digioia, Gina; Izotova, Lara S et al. (2013) Ligand-independent interaction of the type I interferon receptor complex is necessary to observe its biological activity. Cytokine 64:286-97
Wu, Xiangyue; Chesoni, Sandra; Rondeau, Gaelle et al. (2013) Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected target mRNAs. Nucleic Acids Res 41:2644-58
Krause, Christopher D; Izotova, Lara S; Ren, Gwangwen et al. (2011) Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmid. Stem Cell Res Ther 2:15
Matus-Nicodemos, Rodrigo; Vavassori, Stefano; Castro-Faix, Moraima et al. (2011) Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71
Yamaza, T; Ren, G; Akiyama, K et al. (2011) Mouse mandible contains distinctive mesenchymal stem cells. J Dent Res 90:317-24
Sarkar, Srijata; Han, Junfeng; Sinsimer, Kristina S et al. (2011) RNA-binding protein AUF1 regulates lipopolysaccharide-induced IL10 expression by activating IkappaB kinase complex in monocytes. Mol Cell Biol 31:602-15
Knapinska, Anna M; Gratacós, Frances M; Krause, Christopher D et al. (2011) Chaperone Hsp27 modulates AUF1 proteolysis and AU-rich element-mediated mRNA degradation. Mol Cell Biol 31:1419-31

Showing the most recent 10 out of 36 publications