The Protein Chemistry Laboratory Core will serve as the major protein preparation and analysisresource for the Program Project. The laboratory will be responsible for protein preparation,characterization and identification which will include hypothesis and project driven proteomics. TheProtein Chemistry Laboratory is equipped with three automated peptide synthesizers, an automatedEdman sequencer, various HPLC and FPLC systems encompassing both micro and macro scale.Most importantly the laboratory has two 'proteomics-appropriate' mass spectrometry systemsequipped with ionization modes that will enable sub-picomole analysis of proteins and peptides withbio-informatics support. All protein and peptide products will be subjected to stringentcharacterization by mass spectrometric/ proteomic analysis. Surface Plasmon resonance (Biacore)analysis is also available that is adaptable to coupling with the on-site mass spectrometers.The primary services provided will be the provision of protein-related tools and reagents such aspurified murine complement system proteins, monoclonal and polyclonal antibodies, syntheticpeptides, and peptide-like synthetic antagonists that interrupt various and specific complementsystem protein-protein interactions. Hypothesis driven proteomics will also be applied to delineatemolecular and biochemical mechanisms underlying the various complement regulated diseasemodels. All members of the program project will be served by the PCLC.
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