RNA sequencing has quickly evolved to be a revolutionary tool for transcriptomics. Currently, it is one of the most sensitive and comprehensive tools for assessment of transcript abundance and variants in various biological samples. In this application, the RNA-seq Core will perform a vital role in performing transcriptomics and bioinformatic analyses of samples obtained from all projects.
The specific aims of the RNA-seq/Bioinformatics Core are to: 1. Perform RNA sequencing (RNA-seq) services for each of the projects. This includes sequencing of poly A+ RNA to quantify polyadenylated transcripts in tissues. 2. Perform bioinformatic analysis of the RNA-seq data. This would include relative RNA abundance, splice variation, and presence of non-human ORFs. Core Function: The core functions will be described in general terms and then in relation to the specific objectives listed above.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI106684-01A1
Application #
8853015
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2015-06-01
Project End
2020-05-31
Budget Start
2015-06-01
Budget End
2016-05-31
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
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