Abstract

The Human Sample Core C will be responsible for: 1) collection and preparation of clinical samples and lung specimens; 2) evaluation of vaccine candidates in human primary epithelial cells; and 3) measuring serum RSV antibodies from human and cotton rat samples. It will support and interact with all 4 Projects and the Animal Core B included in this P01. Thus, this Core will serve two main goals. First, it will assure the quality of the human samples, and it will simplify streamlining sample organization, data management and compliance with all regulations. Second, this core will be responsible for: a) running the in vitro primary cell culture assays to evaluate vaccine candidates generated by Projects 1, 2 and 3; and b) measuring serum RSV antibodies in serum samples obtained from infants included in Project 4 and from experimental infections in cotton rats performed in the Animal Core B. This approach will optimize the use of resources and will facilitate integration and synergy among all projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI112524-05
Application #
9656059
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
2021-01-31
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
5
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Nationwide Children's Hospital
Department
Type
DUNS #
147212963
City
Columbus
State
OH
Country
United States
Zip Code
43205

  Publications

Hicks, Stephanie N; Chaiwatpongsakorn, Supranee; Costello, Heather M et al. (2018) Five Residues in the Apical Loop of the Respiratory Syncytial Virus Fusion Protein F2 Subunit Are Critical for Its Fusion Activity. J Virol 92:
Ramilo, Octavio; Mejias, Asuncion (2018) Respiratory Syncytial Virus-induced Acute Disease Severity and Long-Term Wheezing. Uncovering the Unexpected. Am J Respir Crit Care Med 198:984-986
Hussain, Syed-Rehan A; Mejias, Asuncion; Ramilo, Octavio et al. (2018) Post-viral atopic airway disease: pathogenesis and potential avenues for intervention. Expert Rev Clin Immunol :1-10
Garcia-Mauriño, Cristina; Moore-Clingenpeel, Melissa; Wallihan, Rebecca et al. (2018) Discharge Criteria for Bronchiolitis: An Unmet Need. Pediatr Infect Dis J 37:514-519
Wang, Yilong; Liu, Rongxian; Lu, Mijia et al. (2018) Enhancement of safety and immunogenicity of the Chinese Hu191 measles virus vaccine by alteration of the S-adenosylmethionine (SAM) binding site in the large polymerase protein. Virology 518:210-220
Li, Anzhong; Yu, Jingyou; Lu, Mijia et al. (2018) A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein. Nat Commun 9:3067
Korppi, Matti (2018) Discharge Criteria for Bronchiolitis: Does Age Matter? Pediatr Infect Dis J 37:e350
Garcia-Mauriño, Cristina; Moore-Clingenpeel, Melissa; Ramilo, Octavio et al. (2018) Re: ""Discharge Criteria for Bronchiolitis: Does Age Matter?"" Pediatr Infect Dis J 37:e350-e351
Grieves, Jessica L; Yin, Zhiwei; Garcia-Sastre, Adolfo et al. (2018) A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats. Vaccine 36:3842-3852
Garcia-Mauriño, Cristina; Moore-Clingenpeel, Melissa; Thomas, Jessica et al. (2018) Viral Load Dynamics and Clinical Disease Severity in Infants with Respiratory Syncytial Virus Infection. J Infect Dis :

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