Abstract

This is a renewal grant application for an ongoing Program Project whose overall objectives have been the study of the chemistry, metabolism and molecular biology of connective tissues. The program is composed of 5 Subprojects and 2 Core Units. Project 1 will continue to investigate the mechanisms that regulate elastin gene expression and elastin fiber formation. Project 2 is a new Subproject; it will explore the mechanisms which regulate Type III collagen gene expression in cartilage. Project 3 will continue to investigate the nature and location of antigenic epitopes on Type IV collagen and examine structural and functional differences between laminins produced by normal and neoplastic cell lines. Project 4 will focus on studies which should explore the mechanisms of the nephritogenic immune response in interstitial nephritis. Project 5 will continue to utilize approaches that should help elucidate the molecular structure of genes which encode new collagen types. An Administrative Core Unit and a Core Laboratory Facility will supplement the research efforts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
5P01AR020553-19
Application #
2078446
Study Section
Arthritis and Musculoskeletal and Skin Diseases Special Grants Review Committee (AMS)
Project Start
1978-05-01
Project End
1997-04-30
Budget Start
1995-06-05
Budget End
1996-04-30
Support Year
19
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University City Science Center
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Myers, Jeanne C; Amenta, Peter S; Dion, Arnold S et al. (2007) The molecular structure of human tissue type XV presents a unique conformation among the collagens. Biochem J 404:535-44
Shahan, Tracy; Grant, Derrick; Tootell, Mason et al. (2004) Oncothanin, a peptide from the alpha3 chain of type IV collagen, modifies endothelial cell function and inhibits angiogenesis. Connect Tissue Res 45:151-63
Myers, Jeanne C; Li, Deqin; Amenta, Peter S et al. (2003) Type XIX collagen purified from human umbilical cord is characterized by multiple sharp kinks delineating collagenous subdomains and by intermolecular aggregates via globular, disulfide-linked, and heparin-binding amino termini. J Biol Chem 278:32047-57
Fawzi, A; Robinet, A; Monboisse, J C et al. (2000) A peptide of the alpha 3(IV) chain of type IV collagen modulates stimulated neutrophil function via activation of cAMP-dependent protein kinase and Ser/Thr protein phosphatase. Cell Signal 12:327-35
Shahan, T A; Fawzi, A; Bellon, G et al. (2000) Regulation of tumor cell chemotaxis by type IV collagen is mediated by a Ca(2+)-dependent mechanism requiring CD47 and the integrin alpha(V)beta(3). J Biol Chem 275:4796-802
Pasco, S; Han, J; Gillery, P et al. (2000) A specific sequence of the noncollagenous domain of the alpha3(IV) chain of type IV collagen inhibits expression and activation of matrix metalloproteinases by tumor cells. Cancer Res 60:467-73
Shahan, T A; Ohno, N; Pasco, S et al. (1999) Inhibition of tumor cell proliferation by type IV collagen requires increased levels of cAMP. Connect Tissue Res 40:221-32
Shahan, T A; Ziaie, Z; Pasco, S et al. (1999) Identification of CD47/integrin-associated protein and alpha(v)beta3 as two receptors for the alpha3(IV) chain of type IV collagen on tumor cells. Cancer Res 59:4584-90
Han, J; Jenq, W; Kefalides, N A (1999) Integrin alpha2beta1 recognizes laminin-2 and induces C-erb B2 tyrosine phosphorylation in metastatic human melanoma cells. Connect Tissue Res 40:283-93
Zhang, Y; Niu, Z; Cohen, A J et al. (1999) The internal chondrocyte-specific promoter of the chick type III collagen gene is activated by AP1 and is repressed in fibroblasts by a complex containing an LBP1-related protein. Nucleic Acids Res 27:4090-9

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