Abstract

Since the primary thrust of this program project centers on keratinocyte differentiation, each of the projects will utilize the Morphology Core for various types of morphological assays. Cultures analyzed biochemically also will be assessed morphologically to correlate functional results with light microscopic and ultrastructural criteria of differentiation. Moreover, the Morphology Core will be used to establish the purity of cell samples and subcellular fractions, as well as the presence of 3T3 cell or fibroblast contamination. Finally, the Morphology Core will utilize its light/phase/fluorescence photomicroscopy unit to assist investigators with immunofluorescence, fluorescence tracer studies (for ionic calcium and proton determinations), and phase contrast monitoring of subcellular fractions and liposome preparations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
5P01AR039448-07
Application #
3747912
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Bikle, Daniel D (2016) Extraskeletal actions of vitamin D. Ann N Y Acad Sci 1376:29-52
Bikle, Daniel D; Oda, Yuko; Tu, Chia-Ling et al. (2015) Novel mechanisms for the vitamin D receptor (VDR) in the skin and in skin cancer. J Steroid Biochem Mol Biol 148:47-51
Bikle, Daniel D (2014) Vitamin D metabolism, mechanism of action, and clinical applications. Chem Biol 21:319-29
Bikle, Daniel D (2014) Vitamin D and cancer: the promise not yet fulfilled. Endocrine 46:29-38
Bikle, Daniel D (2014) The vitamin D receptor: a tumor suppressor in skin. Adv Exp Med Biol 810:282-302
Tu, Chia-Ling; Bikle, Daniel D (2013) Role of the calcium-sensing receptor in calcium regulation of epidermal differentiation and function. Best Pract Res Clin Endocrinol Metab 27:415-27
Jiang, Yan J; Kim, Peggy; Uchida, Yoshikazu et al. (2013) Ceramides stimulate caspase-14 expression in human keratinocytes. Exp Dermatol 22:113-8
Bikle, Daniel D (2012) Vitamin D and the skin: Physiology and pathophysiology. Rev Endocr Metab Disord 13:3-19
Bourguignon, L Y W; Earle, C; Wong, G et al. (2012) Stem cell marker (Nanog) and Stat-3 signaling promote MicroRNA-21 expression and chemoresistance in hyaluronan/CD44-activated head and neck squamous cell carcinoma cells. Oncogene 31:149-60
Tu, Chia-Ling; Crumrine, Debra A; Man, Mao-Qiang et al. (2012) Ablation of the calcium-sensing receptor in keratinocytes impairs epidermal differentiation and barrier function. J Invest Dermatol 132:2350-2359

Showing the most recent 10 out of 223 publications