Core - Tissue Culture and Tissue Analysis

Karasek, Marvin A.

Abstract

The Tissue Culture Core Laboratory was developed to provide stable and consistent technical expertise to investigators of all projects and to accommodate the expected increased need to stabilize the sources of materials required by all. The obvious advantage of the Tissue Culture Core is that of having a skilled technical staff dedicated to a specific activity in a situation in which each patient-derived tissue is precious. Because the Tissue Culture Core facility will grow fibroblasts from the skin of EB patients there will also be an ongoing source of DNA for the linkage studies. This core facility will be under the direction of Dr. Marvin Karasek and the day-to-day tasks of the core will be carried out by Wayne Giomi, technician. The Tissue Analysis Core facility will be limited to a small amount of routine histology and to electron microscopy. Both of these functions will be directed by Dr. Bruce Smoller and performed in the Department of Pathology.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Program Projects (P01)
• Project #: 5P01AR041045-05
• Application #: 5206263
• Support Year: 5
• Fiscal Year: 1996

Publications

Chen, Mei; O'Toole, Edel A; Sanghavi, Jigisha et al. (2002) The epidermolysis bullosa acquisita antigen (type VII collagen) is present in human colon and patients with crohn's disease have autoantibodies to type VII collagen. J Invest Dermatol 118:1059-64

Lara-Pezzi, E; Serrador, J M; Montoya, M C et al. (2001) The hepatitis B virus X protein (HBx) induces a migratory phenotype in a CD44-dependent manner: possible role of HBx in invasion and metastasis. Hepatology 33:1270-81

Yurko, M A; O'Toole, E A; Woodley, D T (2001) Phosphorylation of focal adhesion kinase (pp125(FAK)) is increased in human keratinocytes induced to migrate by extracellular matrices. J Cell Physiol 188:24-32

Wang, C K; Nelson, C F; Brinkman, A M et al. (2000) Spontaneous cell sorting of fibroblasts and keratinocytes creates an organotypic human skin equivalent. J Invest Dermatol 114:674-80

Nasca, M R; O'Toole, E A; Palicharla, P et al. (1999) Thalidomide increases human keratinocyte migration and proliferation. J Invest Dermatol 113:720-4

Chen, M; O'Toole, E A; Li, Y Y et al. (1999) Alpha 2 beta 1 integrin mediates dermal fibroblast attachment to type VII collagen via a 158-amino-acid segment of the NC1 domain. Exp Cell Res 249:231-9

Amieva, M R; Litman, P; Huang, L et al. (1999) Disruption of dynamic cell surface architecture of NIH3T3 fibroblasts by the N-terminal domains of moesin and ezrin: in vivo imaging with GFP fusion proteins. J Cell Sci 112 ( Pt 1):111-25

Chen, M; Marinkovich, M P; Jones, J C et al. (1999) NC1 domain of type VII collagen binds to the beta3 chain of laminin 5 via a unique subdomain within the fibronectin-like repeats. J Invest Dermatol 112:177-83

Nakamura, F; Huang, L; Pestonjamasp, K et al. (1999) Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides. Mol Biol Cell 10:2669-85

Matsui, C; Pereira, P; Wang, C K et al. (1998) Extent of laminin-5 assembly and secretion effect junctional epidermolysis bullosa phenotype. J Exp Med 187:1273-83