The Transgenic mouse/gene targeting core will generate transgenic mice by pronuclear injection of gene constructs carrying specific regulatory sequences that are expressed in cartilage and bone, fused to a reporter gene. The transgenic mouse founders will be delivered to the individual Program investigators for subsequent analysis. Three of the four projects propose to generate transgenic mice with a series of gene constructs. In addition, this core will generate ES cell lines carrying mutations in the cartilage and bone development genes studied by the Program investigators. Gene targeting constructs generated by the Program investigators will be electroporated into mouse ES cells and cultured under selective conditions to enrich for homologous recombination events. The resulting ES colonies will be picked, expanded, and split for temporary freeze and DNA preparation. DNAs will be isolated and restricted with the appropriate enzymes. The restricted DNAs will then be delivered to the Project investigators for subsequent Southern analysis. Once identified, the core will expand targeted clones for freeze down in nitrogen. The core will inject ES clones carrying the targeted mutations into blastocysts to generate mouse chimeras. The resulting mouse chimeras will be delivered to the individual program investigators for subsequent analysis. Each of the four projects proposes to generate mice containing targeted mutations. The Transgenic mouse/gene targeting core represents an essential component of this Program Project.

Project Start
1998-04-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Texas MD Anderson Cancer Center
Department
Type
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030
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