Abstract

The Muscle Physiology Core is a well equipped in vitro physiology laboratory staffed with experienced personnel skilled in executing personnel skilled in executing the proposed contractile studies. This Core will be supervised by Jon Watchko, M.D., and be available to all principal investigators assisting them in achieving their research goals evaluating the effects of gene therapy on skeletal muscle function. In addition to meeting the overlapping needs of individual projects and thereby being the most efficient use of space, equipment and personnel talent, this Core will enhance collaboration between investigators.
The Specific Aims of the Muscle Physiology ore are to i) obtain natural history data on muscle function of the tibialis anterior in normal and dystrophic (dystrophin-deficient mdx) mice, and normal and dystrophic cardiomyopathic) hamsters, and ii) determine the positive or negative effects of viral vector gene delivery on contractile function of the tibialis anterior muscle of dystrophin deficient mice and delta- sarcoglycan hamsters.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Program Projects (P01)
Project #
1P01AR045925-01
Application #
6100729
Study Section
Project Start
1999-04-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Reay, D P; Bilbao, R; Koppanati, B M et al. (2008) Full-length dystrophin gene transfer to the mdx mouse in utero. Gene Ther 15:531-6
Zhang, Jing-Shi; Liu, Feng; Conwell, Christine C et al. (2006) Mechanistic studies of sequential injection of cationic liposome and plasmid DNA. Mol Ther 13:429-37
Bilbao, Roberto; Reay, Daniel P; Li, Juan et al. (2005) Patterns of gene expression from in utero delivery of adenoviral-associated vector serotype 1. Hum Gene Ther 16:678-84
Conwell, Christine C; Huang, Leaf (2005) Recent advances in non-viral gene delivery. Adv Genet 53:3-18
Deasy, B M; Gharaibeh, B M; Pollett, J B et al. (2005) Long-term self-renewal of postnatal muscle-derived stem cells. Mol Biol Cell 16:3323-33
Bilbao, R; Reay, D P; Wu, E et al. (2005) Comparison of high-capacity and first-generation adenoviral vector gene delivery to murine muscle in utero. Gene Ther 12:39-47
Kimura, Shigemi; Ikezawa, Makoto; Cao, Baohong et al. (2004) Ex vivo gene transfer to mature skeletal muscle by using adenovirus helper cells. J Gene Med 6:155-65
Cao, B; Bruder, J; Kovesdi, I et al. (2004) Muscle stem cells can act as antigen-presenting cells: implication for gene therapy. Gene Ther 11:1321-30
Bilbao, Roberto; Reay, Daniel P; Koppanati, Bhanu M et al. (2004) Biocompatibility of adenoviral vectors in poly(vinyl chloride) tubing catheters with presence or absence of plasticizer di-2-ethylhexyl phthalate. J Biomed Mater Res A 69:91-6
Li, Zhenhua; Huang, Leaf (2004) Sustained delivery and expression of plasmid DNA based on biodegradable polyester, poly(D,L-lactide-co-4-hydroxy-L-proline). J Control Release 98:437-46

Showing the most recent 10 out of 52 publications