Abstract

This project seeks to elucidate the mechanisms that regulate the replication and differentiation of normal and cancer cells. The goal is the application of such knowledge to the prevention or treatment of malignant disease in humans. Significant research progress has been made in these specific areas: 1. Studies of the interaction of the transcription machinery with DNA templates using purified RNA polymerases from E. coli and wheat germ and specific cloned fragments of the DNA. 2. Molecular events in the processing of primary RNA transcripts in nuclei of eukaryotic cells. 3. Studies of the role of specific DNA sequences in the control of the promotion, termination, and anti-termination of transcription. 4. The development of genetic systems for studying embryonic development. 5. The molecular processes involved in the replication and assembly of new chromatin. 6. The mechanism of action of estrogen and glucocorticoid receptors in the regulation of gene expression. 7. The role of nuclear and cytoplasmic membranes and the phosphorylation of the constituent membrane proteins in regulation of genetic expression. 8. Studies of the interaction of tumor-promoting phorbol esters and anti-tumorigenic retinoids in the control of cell replication and differentiation. (V)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA023076-09
Application #
3093015
Study Section
(SRC)
Project Start
1978-07-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Thompson, Nancy E; Glaser, Bryan T; Foley, Katherine M et al. (2009) Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB. J Biol Chem 284:24754-66
Thompson, Nancy E; Foley, Katherine M; Burgess, Richard R (2004) Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein and use in immunoaffinity chromatography. Protein Expr Purif 36:186-97
Burgess, R R; Arthur, T M; Pietz, B C (2000) Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins. Methods Enzymol 328:141-57
Bailey, J; Cook, L J; Kilmer-Barber, R et al. (1999) Identification of three genes expressed primarily during development in Physarum polycephalum. Arch Microbiol 172:364-76
Thompson, N E; Burgess, R R (1999) Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF. Protein Expr Purif 17:260-6
Cox, R D; Hugill, A; Shedlovsky, A et al. (1999) Contrasting effects of ENU induced embryonic lethal mutations of the quaking gene. Genomics 57:333-41
Doyle, G A; Betz, N A; Leeds, P F et al. (1998) The c-myc coding region determinant-binding protein: a member of a family of KH domain RNA-binding proteins. Nucleic Acids Res 26:5036-44
McLaren, R S; Caruccio, N; Ross, J (1997) Human La protein: a stabilizer of histone mRNA. Mol Cell Biol 17:3028-36
T'Jampens, D; Meerschaert, K; Constantin, B et al. (1997) Molecular cloning, over-expression, developmental regulation and immunolocalization of fragminP, a gelsolin-related actin-binding protein from Physarum polycephalum plasmodia. J Cell Sci 110 ( Pt 10):1215-26
Ross, J (1997) A hypothesis to explain why translation inhibitors stabilize mRNAs in mammalian cells: mRNA stability and mitosis. Bioessays 19:527-9

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