Abstract

This Program Project deals with experimental leukemogenesis. Callaborative investigations of cellular and virological mechanisms related to leukemogenesis are proposed. The expression of endogenous Type C RNA viruses by lymphocytes in various stages of differentiation will be analyzed and related to the development of leukemia. Neoplastic and normal lymphocytes will be used to identify and analyze cells that are targets of transforming viruses. Virus expression by cells in different stages of malignant transformation will be ascertained. The contribution of lymphocyte differentiation to the formation of recombinant viruses will be determined. The genetic structure of the latter agents will be determined and related to their biological effects. Transforming and non-transforming avian oncornoaviruses will be used to determine structure-function relations of src genets.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA024530-06
Application #
3093085
Study Section
Cancer Special Program Advisory Committee (CAK)
Project Start
1979-05-01
Project End
1987-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Howes, S H; Bockus, B J; Schaffhausen, B S (1996) Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members. J Virol 70:3581-8
Corina, K; Grossman, S R; Barsoum, J et al. (1993) The tryptophan bridge is a critical feature of the papillomavirus E2 DNA binding domain. Virology 197:391-6
Prakash, S S; Grossman, S R; Pepinsky, R B et al. (1992) Amino acids necessary for DNA contact and dimerization imply novel motifs in the papillomavirus E2 trans-activator. Genes Dev 6:105-16
Runnels, J; Serunian, L; Thursby, M et al. (1991) Moloney leukemia virus immortalizes B lymphocytes in vitro. J Virol 65:5549-52
Morrissey, L C; Barsoum, J; Androphy, E J (1989) Trans activation by the bovine papillomavirus E2 protein in Saccharomyces cerevisiae. J Virol 63:4422-5
Zouali, M; Stollar, B D; Schwartz, R S (1988) Origin and diversification of anti-DNA antibodies. Immunol Rev 105:137-59
Schwartz, R S (1988) Polyvalent anti-DNA autoantibodies: immunochemical and biological significance. Int Rev Immunol 3:97-115
Sabbaga, J; Migliorini, P; Schwartz, R S (1988) Autoantibodies to DNA. In Vivo 2:61-5
Hubbert, N L; Schiller, J T; Lowy, D R et al. (1988) Bovine papilloma virus-transformed cells contain multiple E2 proteins. Proc Natl Acad Sci U S A 85:5864-8
Stoye, J P; Coffin, J M (1988) Polymorphism of murine endogenous proviruses revealed by using virus class-specific oligonucleotide probes. J Virol 62:168-75

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