Abstract

Funds are requested to establish a core facility for automated nucleotide sequence analysis. The core will be supervised by Dr. Patrick Hearing, and it will be used by all members of the Program Project. Each project in the Program proposes a wide range of cloning manipulations of wild-type and mutant proteins for expression in mammalian, Sf9 and bacterial cells. All constructs must be verified by nucleotide sequence analysis a time- consuming effort for individual research scientists in our groups. In 1999, we collectively purchased an ABI PRISM 310 Genetic Analyzer for automated sequence analysis. A technician at 50% effort will perform the routine operation of the ABI PRISM 310 Genetic Analyzer as well as prepare miniprep plasmid DNAs and perform the sequencing reactions. The centralizing facility will ensure quality control and produce an economy of effort for the Program members. The availability of centralized nucleotide sequence analysis will enhance each research project in the Program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA028146-21A1
Application #
6542699
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
1980-08-01
Project End
2006-06-30
Budget Start
Budget End
Support Year
21
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Feres, Kimberly J; Hayman, Michael J (2010) RON-expressing MCF-10A breast epithelial cells exhibit alterations of hyaluronan expression, promoting RON-mediated early adhesion events. Biochem Biophys Res Commun 391:1604-9
Feres, K J; Ischenko, I; Hayman, M J (2009) The RON receptor tyrosine kinase promotes MSP-independent cell spreading and survival in breast epithelial cells. Oncogene 28:279-88
Yoo, Jae Cheal; Hayman, Michael J (2007) Annexin II binds to SHP2 and this interaction is regulated by HSP70 levels. Biochem Biophys Res Commun 356:906-11
Tartaglia, Marco; Pennacchio, Len A; Zhao, Chen et al. (2007) Gain-of-function SOS1 mutations cause a distinctive form of Noonan syndrome. Nat Genet 39:75-9
Reich, Nancy C (2007) STAT dynamics. Cytokine Growth Factor Rev 18:511-8
Yondola, Mark A; Hearing, Patrick (2007) The adenovirus E4 ORF3 protein binds and reorganizes the TRIM family member transcriptional intermediary factor 1 alpha. J Virol 81:4264-71
Ullman, Amanda J; Reich, Nancy C; Hearing, Patrick (2007) Adenovirus E4 ORF3 protein inhibits the interferon-mediated antiviral response. J Virol 81:4744-52
Zhao, Chen; Du, Guangwei; Skowronek, Karl et al. (2007) Phospholipase D2-generated phosphatidic acid couples EGFR stimulation to Ras activation by Sos. Nat Cell Biol 9:706-12
Kim, Hong Joo; Taylor, Laura J; Bar-Sagi, Dafna (2007) Spatial regulation of EGFR signaling by Sprouty2. Curr Biol 17:455-61
Freedman, Tanya S; Sondermann, Holger; Friedland, Gregory D et al. (2006) A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A 103:16692-7

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