Abstract

This core provides in vivo model support for the three research projects. Specifically: 1. Support for Projects 1 and 2: Tumor growth delay studies will be carried out in male nude mice bering subcutaneously implanted human prostate carcinoma (PC-3, DU-145, LnCaP or CWR22) or human bladder carcinoma (RT4 or 5637). The animals will be treated with local hyperthermia to the tumor-bearing limb via water bath heating in the presence or absence of thermal sensitizer administration and/or fractionated radiation therapy (daily for 5 days by external beam). The results of these studies will provide an indication of therapeutic efficacy for the findings of Project 2 and provide a basis for translation of potential thermal sensitizes into Project 1. 2. Support for Projects 1 and 3: Large animals (pigs) will be used as a preclinical in vivo system to test the characteristics of new insonation portals using FSUM spherical applicator and urethral thermometry techniques. These animals will be fully instrumented for acquisition and analysis of all temperature and perfusion data, thus allowing calculation of ultrasound power fields, temperature fields, and thermal dose data. The results of these studies will translate into continued instrument development in Project 3 and into clinical protocol development in Project 1.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA031303-12
Application #
6102106
Study Section
Project Start
1998-07-28
Project End
2001-05-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
12
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Hurwitz, Mark D; Hansen, Jorgen L; Prokopios-Davos, Savina et al. (2011) Hyperthermia combined with radiation for the treatment of locally advanced prostate cancer: long-term results from Dana-Farber Cancer Institute study 94-153. Cancer 117:510-6
Wang, XiaoZhe; Khaleque, Md Abdul; Zhao, Mei Juan et al. (2006) Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. J Biol Chem 281:782-91
Hurwitz, Mark D; Kaplan, Irving D; Hansen, Jorgen L et al. (2005) Hyperthermia combined with radiation in treatment of locally advanced prostate cancer is associated with a favourable toxicity profile. Int J Hyperthermia 21:649-56
Calderwood, Stuart K (2005) Regulatory interfaces between the stress protein response and other gene expression programs in the cell. Methods 35:139-48
Calderwood, Stuart K; Theriault, Jimmy R; Gong, Jianlin (2005) How is the immune response affected by hyperthermia and heat shock proteins? Int J Hyperthermia 21:713-6
Calderwood, S K (2005) Evolving connections between molecular chaperones and neuronal function. Int J Hyperthermia 21:375-8
Tang, Dan; Khaleque, Md Abdul; Jones, Ellen L et al. (2005) Expression of heat shock proteins and heat shock protein messenger ribonucleic acid in human prostate carcinoma in vitro and in tumors in vivo. Cell Stress Chaperones 10:46-58
Calderwood, Stuart K; Theriault, Jimmy R; Gong, Jianlin (2005) Message in a bottle: role of the 70-kDa heat shock protein family in anti-tumor immunity. Eur J Immunol 35:2518-27
Ciocca, Daniel R; Calderwood, Stuart K (2005) Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications. Cell Stress Chaperones 10:86-103
Tonkiss, J; Calderwood, S K (2005) Regulation of heat shock gene transcription in neuronal cells. Int J Hyperthermia 21:433-44

Showing the most recent 10 out of 52 publications