Abstract

For investigations in this proposal, expert lymphoma diagnostic skills, immunophenotypic and molecular capabilities, and well-characterized tissue are imperative. Members of the Pathology Core (Core B) will provide the following functions: (1) Pathology Review: Acurate diagnosis of lymphomas will be provided by experienced hematopathologists and molecular pathologists. (2) Tissue banking and distribution: A large number of patient samples are stored, catalogued, and readily retrievable for use in the proposed investigations. The Pathologists will provide selection of appropriate material for the proposed studies from over 15,500 tissue specimens available in our archive. (3) Tissue microarrays (TMA): TMAs are an efficient method for studying protein expression, enabling rapid survey of hundreds of patient samples in a single experiment. TMAs will be used for protein expression studies of newly characterized monoclonal antibodies and novel markers identified by gene expression studies in lymphomas. TMAs will also be used to archive patient samples on an on-going manner as they are accrued into clinical studies described in this proposal. (4) Characterization of new antibodies and testing prognostic models by immunohistochemistry (IHC): IHC with a variety of antibodies with sophisticated methods for their analysis will be provided by members of the Pathology Core. Newly generated antibodies to targets identified by gene expression studies will be characterized using TMAs and IHC techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA034233-26
Application #
8063464
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
26
Fiscal Year
2010
Total Cost
$215,553
Indirect Cost

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Myklebust, June H; Brody, Joshua; Kohrt, Holbrook E et al. (2017) Distinct patterns of B-cell receptor signaling in non-Hodgkin lymphomas identified by single-cell profiling. Blood 129:759-770
Anchang, Benedict; Hart, Tom D P; Bendall, Sean C et al. (2016) Visualization and cellular hierarchy inference of single-cell data using SPADE. Nat Protoc 11:1264-79
Sagiv-Barfi, Idit; Kohrt, Holbrook E; Burckhardt, Laura et al. (2015) Ibrutinib enhances the antitumor immune response induced by intratumoral injection of a TLR9 ligand in mouse lymphoma. Blood 125:2079-86
Behbehani, Gregory K; Samusik, Nikolay; Bjornson, Zach B et al. (2015) Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to Therapy. Cancer Discov 5:988-1003
Levine, Jacob H; Simonds, Erin F; Bendall, Sean C et al. (2015) Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis. Cell 162:184-97
Shroff, Emelyn H; Eberlin, Livia S; Dang, Vanessa M et al. (2015) MYC oncogene overexpression drives renal cell carcinoma in a mouse model through glutamine metabolism. Proc Natl Acad Sci U S A 112:6539-44
Spitzer, Matthew H; Gherardini, Pier Federico; Fragiadakis, Gabriela K et al. (2015) IMMUNOLOGY. An interactive reference framework for modeling a dynamic immune system. Science 349:1259425
Sagiv-Barfi, Idit; Kohrt, Holbrook E K; Czerwinski, Debra K et al. (2015) Therapeutic antitumor immunity by checkpoint blockade is enhanced by ibrutinib, an inhibitor of both BTK and ITK. Proc Natl Acad Sci U S A 112:E966-72
Casey, Stephanie C; Vaccari, Monica; Al-Mulla, Fahd et al. (2015) The effect of environmental chemicals on the tumor microenvironment. Carcinogenesis 36 Suppl 1:S160-83
O'Gorman, William E; Hsieh, Elena W Y; Savig, Erica S et al. (2015) Single-cell systems-level analysis of human Toll-like receptor activation defines a chemokine signature in patients with systemic lupus erythematosus. J Allergy Clin Immunol 136:1326-36

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