Abstract

This Program Project, entitled """"""""Immunoglobulin Variable Region Genes and Proteins"""""""" is a multi-disciplinary research effort focused on the goal of understanding the genetics, expression, and function of variable region genes and proteins in man and in an avian species. Major emphasis is on how somatic mutation plays a role in the generation of antibody diversity. The unifying theme of the individual projects is the analysis of immunoglobulin variable regions. In some instances the studies are on deliberately produced hybridomas, in others, in spontaneous transformations which occur during human malignancies such as chronic lymphatic leukemia and acute lymphatic leukemia. Five projects are proposed, bringing together five independent investigators around three core components. One core laboratory (Core A) is a shared resource that will provide cell culture facilities for all members of the Program Project. Core B will provide molecular biology expertise for all members of the Program Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA044016-02
Application #
3094108
Study Section
(SRC)
Project Start
1987-09-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Denepoux, S; Razanajaona, D; Blanchard, D et al. (1997) Induction of somatic mutation in a human B cell line in vitro. Immunity 6:35-46
Andris, J S; Miller, A B; Abraham, S R et al. (1997) Variable region gene segment utilization in rhesus monkey hybridomas producing human red blood cell-specific antibodies: predominance of the VH4 family but not VH4-21 (V4-34). Mol Immunol 34:237-53
Li, Y; Spellerberg, M B; Stevenson, F K et al. (1996) The I binding specificity of human VH 4-34 (VH 4-21) encoded antibodies is determined by both VH framework region 1 and complementarity determining region 3. J Mol Biol 256:577-89
Andris, J S; Abraham, S R; Pascual, V et al. (1995) The human antibody repertoire: heavy and light chain variable region gene usage in six alloantibodies specific for human HLA class I and class II alloantigens. Mol Immunol 32:1105-22
Xia, Y; Hwang, L Y; Cobb, M H et al. (1994) Products of the TAL2 oncogene in leukemic T cells: bHLH phosphoproteins with DNA-binding activity. Oncogene 9:1437-46
Potter, K N; Li, Y C; Capra, J D (1994) The cross-reactive idiotopes recognized by the monoclonal antibodies 9G4 and LC1 are located in framework region 1 of two non-overlapping subsets of human VH4 family encoded antibodies. Scand J Immunol 40:43-9
Andris, J S; Brodeur, B R; Capra, J D (1993) Molecular characterization of human antibodies to bacterial antigens: utilization of the less frequently expressed VH2 and VH6 heavy chain variable region gene families. Mol Immunol 30:1601-16
Baer, R (1993) TAL1, TAL2 and LYL1: a family of basic helix-loop-helix proteins implicated in T cell acute leukaemia. Semin Cancer Biol 4:341-7
Potter, K N; Li, Y; Pascual, V et al. (1993) Molecular characterization of a cross-reactive idiotope on human immunoglobulins utilizing the VH4-21 gene segment. J Exp Med 178:1419-28
Cai, J; Humphries, C; Lutz, C et al. (1992) Analysis of VH251 gene mutation in chronic lymphocytic leukemia (CLL) and normal B-cell subsets. Ann N Y Acad Sci 651:384-92

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