Abstract

The core represents the most efficient, cost-effective, reliable source of the required samples, which will range from modified oligonucleotides and larger DNA sequences to mouse skin treated with polycyclic aromatic hydrocarbons (PAH) and mouse papillomas. All of th necessary facilities and expertise for the Core are available in the Eppley Institute. In brief, the Core will provide mouse skin treated with PA, cells from mouse skin treated with PAH, HeLa cells treated with PAH, and mouse skin papillomas induced by PAH. In addition, the Core will provide 18-base pair oligonucleotides either containing an apurinic site or modified with PAH or catechol estrogen quinones (CE-Q) and longer defined DNA sequences modified with PAH or CE-Q. Provision of biological preparations by the Core component will expedite the research conducted in the program project grant. In addition, analysis of split samples by collaborators will enhance quality control and will assure that comparison of results obtained by different methods is valid.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA049210-09
Application #
6237046
Study Section
Project Start
1997-05-01
Project End
1998-04-30
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
9
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Nebraska Medical Center
Department
Type
DUNS #
City
Omaha
State
NE
Country
United States
Zip Code
68198

  Publications

Cavalieri, Ercole; Saeed, Muhammad; Zahid, Muhammad et al. (2012) Mechanism of DNA depurination by carcinogens in relation to cancer initiation. IUBMB Life 64:169-79
Zahid, Muhammad; Saeed, Muhammad; Rogan, Eleanor G et al. (2010) Benzene and dopamine catechol quinones could initiate cancer or neurogenic disease. Free Radic Biol Med 48:318-24
Saeed, Muhammad; Rogan, Eleanor; Cavalieri, Ercole (2009) Mechanism of metabolic activation and DNA adduct formation by the human carcinogen diethylstilbestrol: the defining link to natural estrogens. Int J Cancer 124:1276-84
Saeed, Muhammad; Higginbotham, Sheila; Gaikwad, Nilesh et al. (2009) Depurinating naphthalene-DNA adducts in mouse skin related to cancer initiation. Free Radic Biol Med 47:1075-81
Lu, Fang; Zahid, Muhammad; Wang, Cheng et al. (2008) Resveratrol prevents estrogen-DNA adduct formation and neoplastic transformation in MCF-10F cells. Cancer Prev Res (Phila Pa) 1:135-45
Gaikwad, Nilesh W; Yang, Li; Muti, Paola et al. (2008) The molecular etiology of breast cancer: evidence from biomarkers of risk. Int J Cancer 122:1949-57
Chakravarti, Dhrubajyoti; Venugopal, Divya; Mailander, Paula C et al. (2008) The role of polycyclic aromatic hydrocarbon-DNA adducts in inducing mutations in mouse skin. Mutat Res 649:161-78
Rogan, Eleanor (2007) Xenoestrogens, biotransformation, and differential risks for breast cancer. Altern Ther Health Med 13:S112-21
Saeed, Muhammad; Higginbotham, Sheila; Rogan, Eleanor et al. (2007) Formation of depurinating N3adenine and N7guanine adducts after reaction of 1,2-naphthoquinone or enzyme-activated 1,2-dihydroxynaphthalene with DNA. Implications for the mechanism of tumor initiation by naphthalene. Chem Biol Interact 165:175-88
Lu, Fang; Zahid, Muhammad; Saeed, Muhammad et al. (2007) Estrogen metabolism and formation of estrogen-DNA adducts in estradiol-treated MCF-10F cells. The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin induction and catechol-O-methyltransferase inhibition. J Steroid Biochem Mol Biol 105:150-8

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