The macromolecular sequencing/synthesis core facility provides the program project with the ability to determine the partial amino acid sequence of proteins differentially expressed by radiosensitive vs. radioresistant tumor cells. The core will also provide assistance with the development of methods for purification of minute quantities of protein and methods of microsequence analysis using an online HLC PTH identification system. In addition, the core will provide synthetic oligonucleotides for use as probes, antisense modulators, and primers for the analysis of radioresistance.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA052066-02
Application #
3808204
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
Prasad, S; Walent, J; Dritschilo, A (1994) ADP-ribosylation of heterogeneous ribonucleoproteins in HeLa cells. Biochem Biophys Res Commun 204:772-9
Prasad, S; Notario, V; Dritschilo, A (1994) Poly(ADP-ribose) polymerase in HeLa cells--a high resolution two-dimensional gel analysis. Appl Theor Electrophor 4:3-10
Parda, D S; Thraves, P J; Kuettel, M R et al. (1993) Neoplastic transformation of a human prostate epithelial cell line by the v-Ki-ras oncogene. Prostate 23:91-8
Jung, M; Dritschilo, A; Mark, G et al. (1993) Identification of multiple repeat sequences and transcription-related elements within introns 4, 8 and 9 of human Raf-1. Biochem Biophys Res Commun 190:462-9
Kasid, U; Pirollo, K; Dritschilo, A et al. (1993) Oncogenic basis of radiation resistance. Adv Cancer Res 61:195-233
Jung, M; Notario, V; Dritschilo, A (1992) Mutations in the p53 gene in radiation-sensitive and -resistant human squamous carcinoma cells. Cancer Res 52:6390-3
Prasad, S C; Dritschilo, A (1992) High-resolution two-dimensional electrophoresis of nuclear proteins: a comparison of HeLa nuclei prepared by three different methods. Anal Biochem 207:121-8
Briscoe, P R; Jorgensen, T J (1991) Improved RNA isolation from cells in tissue culture using a commercial nucleic acid extractor. Biotechniques 10:594-6