The overall objective of the Support Services Core Component is to facilitate, coordinate and foster research that is conducted under the auspices of each of the three projects. The rationale for proposing a Core Component is that the integration and coordination, which are the hallmarks of a good program project, do not occur spontaneously. A proactive approach is essential. The proposed approach has been proven to be effective during an institutionally sponsored trial period, which has also allowed the applicants to recruit a scientifically trained program coordinator, Ms. Mary Jones. She will assist the Core Component leader, Dr. Russell, in ensuring that the Core contributes maximally to facilitating interactions that will make the projects and their leaders synergistically productive. The Support Services Core Component will meet specific needs in three areas: (i) program coordination and facilitation. The objectives of this subsection of the Core Component are to facilitate interaction between the project leaders and, by minimizing duplication and overlap, to increase the time that each laboratory has for research. The outcome will be increased collaboration and productivity. In addition, the Core will coordinate and negotiate bulk purchases of supplies that can be used in al three of the projects, as well as handle the distribution of such items. Significant savings can be realized, as has been shown during the current trial period. Both the Core Component leader, Dr. Russell, and the program coordinator, Ms. Jones, will have responsibility for coordinating and facilitating activities. (ii) quality control and standardization. The objectives of this subsection of the Core are to provide batches of well characterized reagents and cells required by all three laboratories; to standardize reagents and cells between laboratories, so that interlaboratory data can be compared more reliably; and to act as a repository and distribution center for probes and reagents currently held by individual laboratories, but which would be useful to all; and (iii) clerical support. The objective of this subsection of the Core is to provide routine clerical support to the project leaders. Analysis over the current performance period indicates that requirements here can be met with 20 hours per week. Therefore, it is proposed that an office assistant, Ms. Terri Green, work for the program half time; the other half of her effort will be used and paid for by the Cancer Center. Ms. Green's activities devoted to the Program Project will be supervised by the program coordinator, Ms. Jones. With respect to location, the program coordinator, Ms. Jones, will have a 90 ft(2) office adjacent to the Core leader's. These two offices will be linked through a computer network that will soon be extended to the offices of the other project leaders and to the Core laboratory. The latter contains 400 ft(2) and is adjacent to the laboratories of two of the three project leaders (Morrison and Russell). In addition to laboratory equipment and space, the Core laboratory will include a desk and computer terminal for Ms. Green, the office assistant.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA054474-07
Application #
6102697
Study Section
Project Start
1997-12-01
Project End
1999-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
7
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Kansas
Department
Type
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160
Zhang, Yue H; Murphy, William J; Russell, Stephen W et al. (2005) Serum-dependent potentiation of lipopolysaccharide-induced nitric oxide production is mediated by the events after the transcription of inducible type of nitric oxide synthase. Cell Immunol 234:16-22
Crespo, A; Filla, M B; Russell, S W et al. (2000) Indirect induction of suppressor of cytokine signalling-1 in macrophages stimulated with bacterial lipopolysaccharide: partial role of autocrine/paracrine interferon-alpha/beta. Biochem J 349:99-104
Gao, J J; Filla, M B; Lorsbach, R B et al. (2000) Prolonged exposure of mouse macrophages to IFN-beta suppresses transcription of the inducible nitric oxide synthase gene: altered availability of transcription factor Stat1alpha. Eur J Immunol 30:1551-61
Xia, D; Wang, F; Parmely, M J (2000) Inhibition of nuclear factor-kappab activation in mouse macrophages and the RAW 264.7 cell line by a synthetic adenyl carbocyclic nucleoside. Biochem Pharmacol 60:717-27
David, S A; Awasthi, S K; Balaram, P (2000) The role of polar and facial amphipathic character in determining lipopolysaccharide-binding properties in synthetic cationic peptides. J Endotoxin Res 6:249-56
David, S A; Silverstein, R; Amura, C R et al. (1999) Lipopolyamines: novel antiendotoxin compounds that reduce mortality in experimental sepsis caused by gram-negative bacteria. Antimicrob Agents Chemother 43:912-9
Morrison, D C; Silverstein, R; Luchi, M et al. (1999) Structure-function relationships of bacterial endotoxins. Contribution to microbial sepsis. Infect Dis Clin North Am 13:313-40
Gao, J J; Zuvanich, E G; Xue, Q et al. (1999) Cutting edge: bacterial DNA and LPS act in synergy in inducing nitric oxide production in RAW 264.7 macrophages. J Immunol 163:4095-9
Kielian, T; Nagai, E; Ikubo, A et al. (1999) Granulocyte/macrophage-colony-stimulating factor released by adenovirally transduced CT26 cells leads to the local expression of macrophage inflammatory protein 1alpha and accumulation of dendritic cells at vaccination sites in vivo. Cancer Immunol Immunother 48:123-31
Shnyra, A; Brewington, R; Alipio, A et al. (1998) Reprogramming of lipopolysaccharide-primed macrophages is controlled by a counterbalanced production of IL-10 and IL-12. J Immunol 160:3729-36

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