CORE COMPONENT B: Microscopy and Morphometrics The microscopy core facility has been reorganized under the leadership of a cell biologist and a pathologist to ensure that coverage of the diverse microscopy needs of the three projects are adequately maintained throughout the four years of this grant renewal and to direct the duties of the microscopist hired for this project. Microscopic techniques will be used to: 1) Evaluate the integrity of the diaphragm of SCID mice injected with the various cell lines (Project 2) using scanning electron microscopy; 2) Determine basement membrane invasion of SCID mouse organs and tissue by the different prostate cell lines using transmission electron microscopy; 3) Localize the expression of specific integrins, proteases, and extracellular matrix elements during tissue invasion in SCID mouse using confocal laser microscopy and immunogold probes at the TEM level (Projects 1, 2, & 3); 4) Perform confocal microscopy localization of matrilysin expression in tumors generated by subcutaneous and orthotopic injections in athymic nude mice by co-injection of LNCaP and prostate fibroblast cells (Project 3); 5) Perform video microscopy linear migration assay to evaluate the effects of substrate (laminin 5) on tumor cell migration (Project 1).

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA056666-06
Application #
6300438
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
6
Fiscal Year
2000
Total Cost
$139,797
Indirect Cost
Name
University of Arizona
Department
Type
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721
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