CORE COMPONENT C: Automated combined in situ hybridization (ISH) and immunohistochemistry (IHC) laboratory. The purpose of this core is to provide a facility for automated in situ hybridization and immunohistochemistry which will specifically assist in the successful completion of Projects 1 and 3. Using instrumentation developed at the University of Arizona, the Core Leaders have developed a kinetic-mode automated assay which facilitates rapid combined ISH and IHC assays. With this procedure, reactions can be performed within a 1-3 hour time frame (1). Double labeling IHC and ISH are of benefit: (a) in co- localizing interactive molecules relevant to pathogenesis; (b) in relating RNA message to protein product; (c) in demonstrating unique diagnostic immunoarchitectural features; and (d) in demonstrating clonality of tumor cells. This core will directly impact Project 1 (Specific Aim #1) wherein the question of the importance of alpha3 and alpha6 integrin expression will be examined. It will also aid in Aim 2 where the effect of laminin 5 on hemidesmosome formation will be examined. Core C will support Project 2 in Specific Aims 1 and 2 in which the effect of beta 4 and alpha 6t transfection on invasion will be tested. Core C will support Project 3 by providing both protein and mRNA localization to study the expression pattern of matrilysin in subcutaneous and orthotopic LNCaP tumors in immunodeficient mice.
| Nagle, Raymond B; Algotar, Amit M; Cortez, Connie C et al. (2013) ERG overexpression and PTEN status predict capsular penetration in prostate carcinoma. Prostate 73:1233-40 |
| Sroka, Isis C; Sandoval, Cynthia P; Chopra, Harsharon et al. (2011) Macrophage-dependent cleavage of the laminin receptor ?6?1 in prostate cancer. Mol Cancer Res 9:1319-28 |
| Sroka, Isis C; Anderson, Todd A; McDaniel, Kathy M et al. (2010) The laminin binding integrin alpha6beta1 in prostate cancer perineural invasion. J Cell Physiol 224:283-8 |
| Sroka, Isis C; Pond, Gerald D; Nagle, Raymond B et al. (2009) Human Cell Surface Receptors as Molecular Imaging Candidates for Metastatic Prostate Cancer. Open Prost Cancer J 2:59-66 |
| Ports, Michael O; Nagle, Ray B; Pond, Gerald D et al. (2009) Extracellular engagement of alpha6 integrin inhibited urokinase-type plasminogen activator-mediated cleavage and delayed human prostate bone metastasis. Cancer Res 69:5007-14 |
| Demetriou, Manolis C; Kwei, Kevin A; Powell, Marianne B et al. (2008) Integrin A6 Cleavage in Mouse Skin Tumors. Open Cancer J 2:1-4 |
| Sroka, Isis C; Chen, Man Ling; Cress, Anne E (2008) Simplified purification procedure of laminin-332 and laminin-511 from human cell lines. Biochem Biophys Res Commun 375:410-3 |
| Moran, Carlos M; Garriock, Robert J; Miller, Melanie K et al. (2008) Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle. Cell Motil Cytoskeleton 65:652-61 |
| King, Tamara E; Pawar, Sangita C; Majuta, Lisa et al. (2008) The role of alpha 6 integrin in prostate cancer migration and bone pain in a novel xenograft model. PLoS One 3:e3535 |
| Demetriou, Manolis C; Stylianou, Panayiota; Andreou, Maria et al. (2008) Spatially and temporally regulated alpha6 integrin cleavage during Xenopus laevis development. Biochem Biophys Res Commun 366:779-85 |
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