Immunocytochemistry has been a powerful tool in research in cell biology. It allows the precise localization of individual proteins to cell organelles and--when carried to the electron microscope level--even to specific subcompartments thereof. It provides information on localization of proteins in intact cells and therefore is essential in that it complements cells fractionation and biochemical approaches. To apply immunocytochemical methods at the electron microscope level is technically demanding and requires extensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all member of the Program Project to apply these techniques in their program-related research. Personnel associated with the Core have considerable experience in all major approaches current used in immunocytochemistry involving immunofluorescence, immunoperoxidase techniques, and immunogold labeling of ultrathin cryosections. The Core Director has used these techniques in her research over 15 years, and Mr. McCaffery, the Core's Technical Director, has had over 15 years experiences in this area and has co-authored a number of publications, including 10 during the current funding period using these techniques. During the last four years under the auspices of the Program Project, we have operated the facility as a state-of-the-ar Core to serve program members. The Core has been heavily used by all Project leaders as attested to by the fact that 25 publications have utilized core facilities and/or personnel. Availability of the expertise of the expertise of the core has provided new information that led to new research direction for the Project Leaders.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA058689-09
Application #
6448178
Study Section
Project Start
2001-04-20
Project End
2002-03-31
Budget Start
Budget End
Support Year
9
Fiscal Year
2001
Total Cost
$158,272
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Chen, Yao; Lin, Ping; Qiu, Suimin et al. (2007) Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection. Int J Oncol 30:1137-44
Sciorra, Vicki A; Audhya, Anjon; Parsons, Ainslie B et al. (2005) Synthetic genetic array analysis of the PtdIns 4-kinase Pik1p identifies components in a Golgi-specific Ypt31/rab-GTPase signaling pathway. Mol Biol Cell 16:776-93
Zhong, Qi; Watson, Martin J; Lazar, Cheri S et al. (2005) Determinants of the endosomal localization of sorting nexin 1. Mol Biol Cell 16:2049-57
Audhya, Anjon; Loewith, Robbie; Parsons, Ainslie B et al. (2004) Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton. EMBO J 23:3747-57
Rudge, Simon A; Anderson, Deborah M; Emr, Scott D (2004) Vacuole size control: regulation of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 complex, a PtdIns(3,5)P2-specific phosphatase. Mol Biol Cell 15:24-36
Katzmann, David J; Sarkar, Srimonti; Chu, Tony et al. (2004) Multivesicular body sorting: ubiquitin ligase Rsp5 is required for the modification and sorting of carboxypeptidase S. Mol Biol Cell 15:468-80
Alam, Steven L; Sun, Ji; Payne, Marielle et al. (2004) Ubiquitin interactions of NZF zinc fingers. EMBO J 23:1411-21
Lazar, Cheri S; Cresson, Catherine M; Lauffenburger, Douglas A et al. (2004) The Na+/H+ exchanger regulatory factor stabilizes epidermal growth factor receptors at the cell surface. Mol Biol Cell 15:5470-80
Odorizzi, Greg; Katzmann, David J; Babst, Markus et al. (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116:1893-903
Zheng, Bin; Berrie, Christopher P; Corda, Daniela et al. (2003) GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors. Proc Natl Acad Sci U S A 100:1745-50

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