) Specific Aim 1. To provide packaged viral vectors for laboratory research in the Stem Cell Biology and Transplant Program. Retrovirus vectors and lentivirus vectors will be constructed either in the laboratory of the investigator or in the Vector Core Laboratory. The Vector Core will then use the vector construct DNA in transfections to generate viral vector, also conducting necessary quality control analyses (titer, helper assays), thus providing the final vector product to the investigator for experiments described in each individual section of this Program Project Grant proposal.
Specific Aim 2. To provide clinical-grade retro viral vectors for human trials conducted as a part of the Stem Cell Biology and Transplant Program. Clinical-grade retro viral vector will be produced under cGMP conditions at the Molecular and Cellular Therapeutics Facility, St. Paul Campus of the University of Minnesota, for use in human clinical trials to be carried out as a part of the Stem Cell Biology Research Program. Clinical-grade vectors planned for production include marking vector for human hematopoietic and mesodermal progenitor cells (Projects 2, 3 and 5), vectors transducing drug resistance genes (Project 3), and vectors transducing the human alpha-Liduronidase gene (Project 5).
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| Zeiser, Robert; Blazar, Bruce R (2018) Acute Graft-versus-Host Disease. N Engl J Med 378:586 |
| Blazar, Bruce R; MacDonald, Kelli P A; Hill, Geoffrey R (2018) Immune regulatory cell infusion for graft-versus-host disease prevention and therapy. Blood 131:2651-2660 |
| Rothenberger, Meghan; Wagner, John E; Haase, Ashley et al. (2018) Transplantation of CCR5?32 Homozygous Umbilical Cord Blood in a Child With Acute Lymphoblastic Leukemia and Perinatally Acquired HIV Infection. Open Forum Infect Dis 5:ofy090 |
| Lu, Yunjie; Gao, Ji; Zhang, Shaopeng et al. (2018) miR-142-3p regulates autophagy by targeting ATG16L1 in thymic-derived regulatory T cell (tTreg). Cell Death Dis 9:290 |
| Cichocki, Frank; Wu, Cheng-Ying; Zhang, Bin et al. (2018) ARID5B regulates metabolic programming in human adaptive NK cells. J Exp Med 215:2379-2395 |
| Taraseviciute, Agne; Tkachev, Victor; Ponce, Rafael et al. (2018) Chimeric Antigen Receptor T Cell-Mediated Neurotoxicity in Nonhuman Primates. Cancer Discov 8:750-763 |
| Felices, Martin; Lenvik, Alexander J; McElmurry, Ron et al. (2018) Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect. JCI Insight 3: |
| Sarhan, Dhifaf; Hippen, Keli L; Lemire, Amanda et al. (2018) Adaptive NK Cells Resist Regulatory T-cell Suppression Driven by IL37. Cancer Immunol Res 6:766-775 |
| Williams, Robin L; Cooley, Sarah; Bachanova, Veronika et al. (2018) Recipient T Cell Exhaustion and Successful Adoptive Transfer of Haploidentical Natural Killer Cells. Biol Blood Marrow Transplant 24:618-622 |
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