Abstract

The primary objective of the mRNA Analysis Facility is the development and employment of automated instrumentation to support the tissue analysis requirements of the project. Both capillary dispensing system for printing the DNA microassays within conventional 96 well microplates as well as an analysis workstation containing automated sample preparation, hybridization, washing, detection and information processing will be constructed for routine analysis of the lung tissue samples provided by the UT collaborators (Core B). Deliverables include a CCD proximal detector/imager to be utilized in the final workstation which will first undergo independent evaluation in a clinical environment at NCI.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA074173-03
Application #
6300535
Study Section
Project Start
2000-02-14
Project End
2002-01-10
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$252,885
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Wang, X; Wang, L; Callister, M D et al. (2001) Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues. Cancer Res 61:7417-21
Soria, J C; Moon, C; Wang, L et al. (2001) Effects of N-(4-hydroxyphenyl)retinamide on hTERT expression in the bronchial epithelium of cigarette smokers. J Natl Cancer Inst 93:1257-63
Jang, S J; Soria, J C; Wang, L et al. (2001) Activation of melanoma antigen tumor antigens occurs early in lung carcinogenesis. Cancer Res 61:7959-63
Wu, W G; Soria, J C; Wang, L et al. (2000) TRAIL-R2 is not correlated with p53 status and is rarely mutated in non-small cell lung cancer. Anticancer Res 20:4525-9
Soria, J C; Jang, S J; Khuri, F R et al. (2000) Overexpression of cyclin B1 in early-stage non-small cell lung cancer and its clinical implication. Cancer Res 60:4000-4
Jang, S J; Mao, L (2000) Methylation patterns in human androgen receptor gene and clonality analysis. Cancer Res 60:864-6
Wang, L; Lin, S H; Wu, W G et al. (2000) C-CAM1, a candidate tumor suppressor gene, is abnormally expressed in primary lung cancers. Clin Cancer Res 6:2988-93
Jing, N; De Clercq, E; Rando, R F et al. (2000) Stability-activity relationships of a family of G-tetrad forming oligonucleotides as potent HIV inhibitors. A basis for anti-HIV drug design. J Biol Chem 275:3421-30
Wu, W; Kemp, B L; Proctor, M L et al. (1999) Expression of DMBT1, a candidate tumor suppressor gene, is frequently lost in lung cancer. Cancer Res 59:1846-51
Wu, W; Fan, Y H; Kemp, B L et al. (1998) Overexpression of cdc25A and cdc25B is frequent in primary non-small cell lung cancer but is not associated with overexpression of c-myc. Cancer Res 58:4082-5