The Radiation Core will provide the irradiation in support of all of the projects in the program. This core is housed in the Lombardi Cancer Center and currently consists of a J.L Shepard Mark I, Model 30, Cesium Irradiator. This Mark I is specifically designed for experimental gamma-irradiations. It contains a 2,200 Ci 137 Cs source. Tissue culture flasks are irradiated by stacking them on the rotating platform. A special rotating tube holder can accommodate test tubes with volumes up to 50 ml. A pie-shaped rotating mouse holder allows simultaneous whole-body irradiations of a dozen mice at a time. The cesium irradiator is suitable for the majority of the irradiations required by this program; however, the inability to effectively focus and block the beam makes it difficult to use the instrument for partial body irradiations, such as tumor irradiations in the flanks of mice. Also, the penetrability of the radiation cannot be controlled because the photon energy cannot be modulated. For these reasons, we are requesting the purchase of a low cost, portable, x-ray machine, that cannot be dedicated to the experimental work of the program. The requested x-ray machine is the RS2000, manufactured by Rad-Source, a division of Microfocus Imaging, Inc of Wheeling, IL. Dr. Timothy Jorgenson is Director of the Radiation Core. His responsibilities include training personnel in the proper and safe use of the instruments, and ensuring compliance with NRC regulations. Dr. Jorgenson chairs an Internal Advisory Committee. The Committee meets as needed to set rules and policies from instrument use, and to make decisions concerning maintenance and repairs. The committee guarantees fair and equitable use of the instruments among the investigators.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA074175-04
Application #
6300544
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
4
Fiscal Year
2000
Total Cost
$199,491
Indirect Cost
Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
Sakabe, Isamu; Hu, Rong; Jin, Lu et al. (2015) TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling. Breast Cancer Res Treat 153:285-97
Zhang, Chuanbo; Kallakury, Bhaskar V; Ross, Jeffrey S et al. (2013) The significance of TNFAIP8 in prostate cancer response to radiation and docetaxel and disease recurrence. Int J Cancer 133:31-42
Cheema, Amrita K; Varghese, Rency S; Timofeeva, Olga et al. (2013) Functional proteomics analysis to study ATM dependent signaling in response to ionizing radiation. Radiat Res 179:674-683
Jung, Mira; Timofeeva, Olga; Cheema, Amrita K et al. (2011) Human fibroblasts for large-scale ""omics"" investigations of ATM gene function. Adv Exp Med Biol 720:181-90
Cheema, Amrita K; Timofeeva, Olga; Varghese, Rency et al. (2011) Integrated analysis of ATM mediated gene and protein expression impacting cellular metabolism. J Proteome Res 10:2651-7
Konsoula, Zacharoula; Velena, Alfredo; Lee, Rachel et al. (2011) Histone deacetylase inhibitor: antineoplastic agent and radiation modulator. Adv Exp Med Biol 720:171-9
Hu, Zhang-Zhi; Huang, Hongzhan; Wu, Cathy H et al. (2011) Omics-based molecular target and biomarker identification. Methods Mol Biol 719:547-71
Varghese, Rency S; Cheema, Amrita; Cheema, Prabhdeep et al. (2010) Analysis of LC-MS data for characterizing the metabolic changes in response to radiation. J Proteome Res 9:2786-93
Broustas, Constantinos G; Ross, Jeffrey S; Yang, Qifeng et al. (2010) The proapoptotic molecule BLID interacts with Bcl-XL and its downregulation in breast cancer correlates with poor disease-free and overall survival. Clin Cancer Res 16:2939-48
Agbor-Enoh, S; Seudieu, C; Davidson, E et al. (2009) Novel inhibitor of Plasmodium histone deacetylase that cures P. berghei-infected mice. Antimicrob Agents Chemother 53:1727-34

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