The immunology Core is Designed to provide immunologic reagents needed in Project 1 through 4. These reagents are established: monoclonal antibodies (Mabs) to the human colorectal carcinoma (CRC) antigen (Ag) GA733 and the murine homolog mEGP. Polyclonal antibodies to these Ags will be purified on Ag immunoaffinity columns. New Mab to fragments of the GA733 Ag (derived from Project 1) will be generated. Antibodies from vaccinating (under Project 3) patients, healthy individuals and patients with ulcerative colitis, will be affinity-purified for detailed tissue binding specificity analysis. In addition, Core B will produce purified baculovirus-derived GA733 Ag and mEGP. A clinical grade batch of baculovirus-derived GA733 Ag will be produced for skin testing of patients in Project 3. All reagants will be tested for purity, identity, and immunoreactivity. The centralized services provided by experienced investigators will improve quality of the Ag and antibody preparations and save overall costs as compared to their inclusion as part of each project.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA074294-01A1
Application #
6269844
Study Section
Project Start
1998-01-07
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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