This core will provide tissue culture-derived reagents for all four projects. The core will make and titer stocks of mutant and wt forms of v-abl and bcr-abl viruses. The core will also use these viruses to transform murine bone marrow cells and fibroblast ell lines in vitro and to infect mice in vivo. The core will also obtain from different projects wt and mutant forms of Jak and Abi-1 proteins and will generate stably transfected lines expressing these proteins in an inducible manner. The core will generate monoclonal antibodies and will provide routine cell culture and freezing for all of the projects.
| Limnander, Andre; Danial, Nika N; Rothman, Paul B (2004) v-Abl signaling disrupts SOCS-1 function in transformed pre-B cells. Mol Cell 15:329-41 |
| Fan, P D; Goff, S P (2000) Abl interactor 1 binds to sos and inhibits epidermal growth factor- and v-Abl-induced activation of extracellular signal-regulated kinases. Mol Cell Biol 20:7591-601 |
| Cong, F; Spencer, S; Cote, J F et al. (2000) Cytoskeletal protein PSTPIP1 directs the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation. Mol Cell 6:1413-23 |
| Cong, F; Yuan, B; Goff, S P (1999) Characterization of a novel member of the DOK family that binds and modulates Abl signaling. Mol Cell Biol 19:8314-25 |
| Cong, F; Goff, S P (1999) c-Abl-induced apoptosis, but not cell cycle arrest, requires mitogen-activated protein kinase kinase 6 activation. Proc Natl Acad Sci U S A 96:13819-24 |
| Cong, F; Zou, X; Hinrichs, K et al. (1999) Inhibition of v-Abl transformation by p53 and p19ARF. Oncogene 18:7731-9 |
