The primary goals of this project are: (i) to serve as a central resource for physical mapping reagents and information to support the functional studies of the other individual projects, and (ii) to utilize and build on these resources to construct a complete PAC/BAC-based map of the distal chromosome 11 region that can ultimately serve as a basis for complete nucleotide sequencing. The laboratory will provide mapping support at several levels: (i) an extensive set of oligonucleotides corresponding both to polymorphic STS's and known genes will be maintained to assist in market content mapping, (ii) low resolution YAC physical maps of distal 11 will initially be assembled through a combination of compiling available mapping data and supplementation by in-house screening, and STS or probe content mapping, (iii) available markers will be used in a hybridization based screening protocols to identify PAC/BAC clones at a projected 10x- average depth of coverage, (iv) additional STSs will be generated by sequencing the ends of PAC and BAC clones and selecting appropriate primer sequences to generate a high density of markers for additional clone identification by a multiplex walking strategy and (v) automated restriction fragment fingerprinting analysis will be conducted with individual genomic clones to generate a contiguous high-resolution PAC/BAC map that will enable identification of a minimal tiling path for DNA sequencing.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA075719-03
Application #
6318320
Study Section
Project Start
2000-06-01
Project End
2001-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$455,266
Indirect Cost
Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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Lorenzetti, Diego; Antalffy, Barbara; Vogel, Hannes et al. (2004) The neurological mutant quaking(viable) is Parkin deficient. Mamm Genome 15:210-7
Clark, Amander T; Firozi, Karen; Justice, Monica J (2004) Mutations in a novel locus on mouse chromosome 11 resulting in male infertility associated with defects in microtubule assembly and sperm tail function. Biol Reprod 70:1317-24
Walz, Katherina; Spencer, Corinne; Kaasik, Krista et al. (2004) Behavioral characterization of mouse models for Smith-Magenis syndrome and dup(17)(p11.2p11.2). Hum Mol Genet 13:367-78
Yan, Jiong; Keener, Victoria W; Bi, Weimin et al. (2004) Reduced penetrance of craniofacial anomalies as a function of deletion size and genetic background in a chromosome engineered partial mouse model for Smith-Magenis syndrome. Hum Mol Genet 13:2613-24
Hentges, Kathryn E; Justice, Monica J (2004) Checks and balancers: balancer chromosomes to facilitate genome annotation. Trends Genet 20:252-9
Yan, Jiong; Walz, Katherina; Nakamura, Hisashi et al. (2003) COP9 signalosome subunit 3 is essential for maintenance of cell proliferation in the mouse embryonic epiblast. Mol Cell Biol 23:6798-808
Walz, Katherina; Caratini-Rivera, Sandra; Bi, Weimin et al. (2003) Modeling del(17)(p11.2p11.2) and dup(17)(p11.2p11.2) contiguous gene syndromes by chromosome engineering in mice: phenotypic consequences of gene dosage imbalance. Mol Cell Biol 23:3646-55
Kile, Benjamin T; Mason-Garrison, Cammy L; Justice, Monica J (2003) Sex and strain-related differences in the peripheral blood cell values of inbred mouse strains. Mamm Genome 14:81-5

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