The Transgenic and Knockout Animal Core is designed to provide a centralized, service oriented facility that will insure the capacity of each participating Program Project Laboratory to produce transgenic and gene-ablated mice. The Core will provide the state-of-the-art facilities and the highly skilled technical personnel required to efficiently and economically generate and maintain animals expressing either targeted genes or specific genes which have been genetically deregulated or ablated in the germline. We have assembled an experienced team responsible for injecting targeted ES cell lines into blastocysts for the production of chimeric mice, establishing breeding programs to produce germline transmission of targeted genes, maintaining and backcrossing individual colonies and preserving established lines by embryo freezing. This technology is crucial to the Program Project's goals and continues to be a major avenue for collaborations.
| Matsui, Ken; O'Mara, Leigh A; Allen, Paul M (2003) Successful elimination of large established tumors and avoidance of antigen-loss variants by aggressive adoptive T cell immunotherapy. Int Immunol 15:797-805 |
| Krug, Anne; Uppaluri, Ravindra; Facchetti, Fabio et al. (2002) IFN-producing cells respond to CXCR3 ligands in the presence of CXCL12 and secrete inflammatory chemokines upon activation. J Immunol 169:6079-83 |
| Dunn, Gavin P; Bruce, Allen T; Ikeda, Hiroaki et al. (2002) Cancer immunoediting: from immunosurveillance to tumor escape. Nat Immunol 3:991-8 |
| Ikeda, Hiroaki; Old, Lloyd J; Schreiber, Robert D (2002) The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev 13:95-109 |
| Hanson, H L; Donermeyer, D L; Ikeda, H et al. (2000) Eradication of established tumors by CD8+ T cell adoptive immunotherapy. Immunity 13:265-76 |
