Abstract

The objective of these experiments is to understand the mechanisms by which Epstein-Barr Virus (EBV) establishes latent infection, persists, and causes neoplasia.
The specific aims are (i) To complete the identification of EBV reading frames and proteins that are expressed at various stages of experimental latent or lytic infection of cells, in vitro. (ii) To identify cellular genes whose expression is specifically altered during the course of latent or lytic EBV infection, in vitro. (iii) To further identify viral and cellular genes expressed in cell lines and tumor tissue from patents with EBV associated Lymphoproliferative Disease, Burkitt's Lymphoma, Hodgkin's Disease, Nasopharyngeal Carcinoma, and Gastric Cancer and to further explore the association of EBV with testicular cancers. (iv) To further identify EBV and cellular gene expression in latently infected lymphocytes in the peripheral blood and lymphoid organs of normal people undergoing phlebotomy, biopsy, or surgical resection. (v) To establish and maintain databases of the effects of EBV on cell gene expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA087661-02
Application #
6484152
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2001-08-01
Project End
2002-07-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Princeton University
Department
Type
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544

  Publications

Kramer, Tal; Greco, Todd M; Taylor, Matthew P et al. (2012) Kinesin-3 mediates axonal sorting and directional transport of alphaherpesvirus particles in neurons. Cell Host Microbe 12:806-14
Lee, Sungwook; Sakakibara, Shuhei; Maruo, Seiji et al. (2009) Epstein-Barr virus nuclear protein 3C domains necessary for lymphoblastoid cell growth: interaction with RBP-Jkappa regulates TCL1. J Virol 83:12368-77
Sciortino, Maria Teresa; Taddeo, Brunella; Giuffre-Cuculletto, Maria et al. (2007) Replication-competent herpes simplex virus 1 isolates selected from cells transfected with a bacterial artificial chromosome DNA lacking only the UL49 gene vary with respect to the defect in the UL41 gene encoding host shutoff RNase. J Virol 81:10924-32
Paulus, Christina; Sollars, Patricia J; Pickard, Gary E et al. (2006) Transcriptome signature of virulent and attenuated pseudorabies virus-infected rodent brain. J Virol 80:1773-86
Zhao, Bo; Maruo, Seiji; Cooper, Andrew et al. (2006) RNAs induced by Epstein-Barr virus nuclear antigen 2 in lymphoblastoid cell lines. Proc Natl Acad Sci U S A 103:1900-5
Poon, Alice P W; Gu, Haidong; Roizman, Bernard (2006) ICP0 and the US3 protein kinase of herpes simplex virus 1 independently block histone deacetylation to enable gene expression. Proc Natl Acad Sci U S A 103:9993-8
Brukman, Alla; Enquist, L W (2006) Pseudorabies virus EP0 protein counteracts an interferon-induced antiviral state in a species-specific manner. J Virol 80:10871-3
Rosendorff, Adam; Sakakibara, Shuhei; Lu, Sixin et al. (2006) NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression. Proc Natl Acad Sci U S A 103:5308-13
Yuan, Jing; Cahir-McFarland, Ellen; Zhao, Bo et al. (2006) Virus and cell RNAs expressed during Epstein-Barr virus replication. J Virol 80:2548-65
Taddeo, Brunella; Roizman, Bernard (2006) The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A. J Virol 80:9341-5

Showing the most recent 10 out of 74 publications