Many of the experimental protocols for the projects in this program rely on retroviral transduction of primaryor cultured cells. Because the techniques of retroviral preparation and transduction require a high degree oftechnical expertise, a central facility where these techniques are performed would significantly enhance theprogress of all projects. A central facility for retroviral preparation and transduction would offer multipleadvantages to the group. These include: 1) uniform procedures for preparing high titer retroviralsupernatants, 2) regular availability of high titer retroviral supernatants, 3) periodic monitoring of retroviralsupernatants to insure that they are mycoplasma-free and do not contain replication competent retrovirus,and 4) expertise in transduction of fetal liver and bone marrow hematopoietic stem cells, Because of thecollaborative nature of the research program, availability of high titer retroviral supernatants and proceduresfor transduction, together with standardization of protocols and reagents would be of great benefit toeveryone in the program. The retroviral core will be housed in Dr. Pear's research space. The Core willpurchase and maintain equipment necessary for these procedures. Additionally, consumables will also bepurchased in bulk by the Core to ensure standardization of materials and bulk purchase discounts.
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