Abstract

The Mouse Molecular Genetics Core will support the components of this Program to facilitate the investigation of the developmental, physiological, and pathological roles of the signaling molecules Bit1, DOCK180, Gab1, and SHEP1. The conditional knockout mouse models generated by each laboratory will be tested for extraembryonic tissue function during development. In addition, each will be tested with appropriate transgenic mouse tumor models. Using a newly developed model based on the K18 gene and the Polyoma Middle T antigen (PyMT) oncogene, the roles of the Bit1, DOCK180, Gab1, and SHEP1 genes in the development of mammary tumors, hepatomas, pancreatic tumors, and prostate neoplasms will be evaluated. In addition, this Core facility will provide investigators with trophoblast stem cells, embryonic fibroblasts, and tumor cells for additional experiments. Finally, this Core will assist and train investigators in embryo development and additional genetic manipulations of their respective genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA102583-05
Application #
7683133
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
5
Fiscal Year
2008
Total Cost
$359,994
Indirect Cost

  Institution

Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Feng, Haizhong; Hu, Bo; Jarzynka, Michael J et al. (2012) Phosphorylation of dedicator of cytokinesis 1 (Dock180) at tyrosine residue Y722 by Src family kinases mediates EGFRvIII-driven glioblastoma tumorigenesis. Proc Natl Acad Sci U S A 109:3018-23
Feng, Haizhong; Hu, Bo; Liu, Kun-Wei et al. (2011) Activation of Rac1 by Src-dependent phosphorylation of Dock180(Y1811) mediates PDGFR?-stimulated glioma tumorigenesis in mice and humans. J Clin Invest 121:4670-84
Mace, Peter D; Wallez, Yann; Dobaczewska, Ma?gorzata K et al. (2011) NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling. Nat Struct Mol Biol 18:1381-7
Múnera, Jorge; Ceceña, Grace; Jedlicka, Paul et al. (2011) Ets2 regulates colonic stem cells and sensitivity to tumorigenesis. Stem Cells 29:430-9
Browne, Cecille D; Hoefer, Melanie M; Chintalapati, Suresh K et al. (2010) SHEP1 partners with CasL to promote marginal zone B-cell maturation. Proc Natl Acad Sci U S A 107:18944-9
Murai, Keith K; Pasquale, Elena B (2010) Restraining stem cell niche plasticity: a new talent of Eph receptors. Cell Stem Cell 7:647-8
Roselli, Séverine; Wallez, Yann; Wang, Lei et al. (2010) The SH2 domain protein Shep1 regulates the in vivo signaling function of the scaffolding protein Cas. Cell Signal 22:1745-52
Pasquale, Elena B (2010) Eph receptors and ephrins in cancer: bidirectional signalling and beyond. Nat Rev Cancer 10:165-80
Wang, Lei; Vervoort, Virginie; Wallez, Yann et al. (2010) The SRC homology 2 domain protein Shep1 plays an important role in the penetration of olfactory sensory axons into the forebrain. J Neurosci 30:13201-10
Leone, Marilisa; Cellitti, Jason; Pellecchia, Maurizio (2009) The Sam domain of the lipid phosphatase Ship2 adopts a common model to interact with Arap3-Sam and EphA2-Sam. BMC Struct Biol 9:59

Showing the most recent 10 out of 34 publications