The Program makes extensive use of human cell lines and animal models of prostate cancer. The overall goal ofthis Core is to provide materials, instructional support, technical aid,/n vivo imaging of mouse models of prostatecancer metastases, and ex-vivo assays of tumor-bone interactions to Project investigators. This will encompassthe storage and distribution of well-characterized prostate cell lines and xenografts; the storage and distribution ofreporter and inducible cell lines; and instruction, guidelines and assistance with xenograft studies, Xenogen andFaxitron imaging and assays for osteoblast, osteoclast and angiogenesis. Such standardization of cell culturereagents and animal techniques will facilitate research by improving the quality of the data, reducing redundancyand allowing comparison of results between the all Projects. This Core will have 5 Aims: 1) Provide wellcharacterizedprostate cancer and normal prostate cell lines to investigators and house a repository of all commontumor and immortalized cell lines. In addition, the Core will carry out mycoplasma testing and geneticfingerprinting (SNP analysis and/or VNTRs) of all cell lines received by Program investigators from outsidesources. Thus, any cell line received by an investigator will first be processed by the Core in this way and asample frozen in the repository. The Core will also serve as a repository of new cell lines derived by Programinvestigators such as those with inducible promoters (S. Parsons, Weber) or with imaging capability such as thosecontaining transfected luciferase constructs (Theodorescu). 2) Provide live instruction and guidelines forinvestigators using xenograft models such as those derived after subcutaneous, intracardiac, intrafemoral andintraprostatic injection of tumor cells and with procedures such as orchiectomy. Instructional videos will be madeof these procedures and saved on a central server database (PROSTA, Core A) accessible by all Programmembers via standard networked PCs. 3) Support for in vivo fluorescent imaging of tumors and Faxitronradiographic imaging and quantitation of bone metastases will be provided by the Core. Technical help will involveproviding instruction as well as taking animals to the UVA SAMMIC core for MS (Xenogen Corp.) imaging oftumors expressing luciferase. 4) Assessment of tumor-bone interactions will be performed using wellcharacterizedassays of osteoblast, osteoclast and endothelial cell function. 5) Microscopy Imaging: a) Resourcesfor the routine analysis and documentation of cell structure and morphology and the detection of macromoleculesin fixed, live cells and tissues; b) resources for single cell analysis; and c) training for Program personnel andoverall oversight of Core equipment.
| Kuscu, Canan; Kumar, Pankaj; Kiran, Manjari et al. (2018) tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner. RNA 24:1093-1105 |
| Hao, Yi; Bjerke, Glen A; Pietrzak, Karolina et al. (2018) TGF? signaling limits lineage plasticity in prostate cancer. PLoS Genet 14:e1007409 |
| Yang, Chun-Song; Melhuish, Tiffany A; Spencer, Adam et al. (2017) The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. Prostate 77:1452-1467 |
| Kumar, Pankaj; Kuscu, Canan; Dutta, Anindya (2016) Biogenesis and Function of Transfer RNA-Related Fragments (tRFs). Trends Biochem Sci 41:679-689 |
| Agarwal, Neeraj; Dancik, Garrett M; Goodspeed, Andrew et al. (2016) GON4L Drives Cancer Growth through a YY1-Androgen Receptor-CD24 Axis. Cancer Res 76:5175-85 |
| Reon, Brian J; Dutta, Anindya (2016) Biological Processes Discovered by High-Throughput Sequencing. Am J Pathol 186:722-32 |
| Sakurai, Kouhei; Reon, Brian J; Anaya, Jordan et al. (2015) The lncRNA DRAIC/PCAT29 Locus Constitutes a Tumor-Suppressive Nexus. Mol Cancer Res 13:828-38 |
| Dillon, Laura W; Kumar, Pankaj; Shibata, Yoshiyuki et al. (2015) Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity. Cell Rep 11:1749-59 |
| Kumar, Pankaj; Mudunuri, Suresh B; Anaya, Jordan et al. (2015) tRFdb: a database for transfer RNA fragments. Nucleic Acids Res 43:D141-5 |
| Earl, Julie; Rico, Daniel; Carrillo-de-Santa-Pau, Enrique et al. (2015) The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies. BMC Genomics 16:403 |
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