Services provided by the Tissue Analysis Core Laboratory are technical and professional.The Core is vital to theProgram Project as all of the individual Projects will utilize the services and resources of the Core. The servicesinclude:
Aim 1. Collection and analysis of Prostate Tumor tissue and cellular samples.
Aim 1. 1) the continued collection of frozen normal and neoplastic prostate tissue specimens from radicalprostatectomies in an appropriate manner to ensure optimal nucleic acid and protein preservation. This isperformed in a manner such that patient care is not compromised.
Aim 1. 2) the performance of routine histology on cell culture, xenograft, mouse models, and clinical humanprostate tissue specimens. In addition, Dr. Frierson will use his expertise as a pathologist to examine theprostate glands of transgenic mice to determine histopathologic changes induced by the genetic modifications.
Aim 1. 3) the performance of immunohistochemistry and the characterization of antibodies, to determine theirutility in frozen tissue sections and in formalin-fixed, paraffin-embedded material with and without usingmethods of epitope retrieval. Dr. Frierson will use his immunohistochemical expertise to determine optimalreagent conditions and antibody staining in cell culture, xenograft, mouse models, and clinical human prostatecancer specimensAim 1.4) selection, preparation, and procurement of cells for laser microdissection. In prostates of transgenicmice, Dr. Frierson will perform laser microdissection of selected cells for molecular analysis.
Aim 2. Bone histology and histomorphometry will be performed on xenograft mouse models of prostatecancer. Bone samples will be processed and analyzed by Dr. Guise to assess the bone metastases phenotypeand to quantitate tumor burden, and osteoclast and osteoblast activity.All of the data generated by CORE C will be submitted to the PROSTA interactive searchable database that willbe designed by CORE A. Submission of data from CORE C to this common data repository and retrieval systemwill better integrate the programmatic interactions between CORE C and all Projects and Cores.
| Kuscu, Canan; Kumar, Pankaj; Kiran, Manjari et al. (2018) tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner. RNA 24:1093-1105 |
| Hao, Yi; Bjerke, Glen A; Pietrzak, Karolina et al. (2018) TGF? signaling limits lineage plasticity in prostate cancer. PLoS Genet 14:e1007409 |
| Yang, Chun-Song; Melhuish, Tiffany A; Spencer, Adam et al. (2017) The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. Prostate 77:1452-1467 |
| Agarwal, Neeraj; Dancik, Garrett M; Goodspeed, Andrew et al. (2016) GON4L Drives Cancer Growth through a YY1-Androgen Receptor-CD24 Axis. Cancer Res 76:5175-85 |
| Reon, Brian J; Dutta, Anindya (2016) Biological Processes Discovered by High-Throughput Sequencing. Am J Pathol 186:722-32 |
| Kumar, Pankaj; Kuscu, Canan; Dutta, Anindya (2016) Biogenesis and Function of Transfer RNA-Related Fragments (tRFs). Trends Biochem Sci 41:679-689 |
| Sakurai, Kouhei; Reon, Brian J; Anaya, Jordan et al. (2015) The lncRNA DRAIC/PCAT29 Locus Constitutes a Tumor-Suppressive Nexus. Mol Cancer Res 13:828-38 |
| Dillon, Laura W; Kumar, Pankaj; Shibata, Yoshiyuki et al. (2015) Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity. Cell Rep 11:1749-59 |
| Kumar, Pankaj; Mudunuri, Suresh B; Anaya, Jordan et al. (2015) tRFdb: a database for transfer RNA fragments. Nucleic Acids Res 43:D141-5 |
| Earl, Julie; Rico, Daniel; Carrillo-de-Santa-Pau, Enrique et al. (2015) The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies. BMC Genomics 16:403 |
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