The primary purpose of the Molecular Profiling and Pathology (MPP) core is to provide the investigators inthis P01 with a centralized and comprehensive facility for microarray-based molecular profiling, genotyping(mutation analysis), and morphology-based studies (histopathology, immunohistochemistry, in situhybridization). The Core will provide expertise in the experimental design and performance of prospectivemicroarray-based molecular profiling studies, and serve as the repository of a large existing integratedgenomics data set of human lung adenocarcinomaswith data on global gene expression profiles, gene copynumber and extensive clinical, pathologic, and mutational annotation. This rich data set is available to bequeried by all research projects for the expression or copy number status of genes of biological ortherapeutic interest and their correlation with other data. Furthermore, the samples will be available as frozentissues, nucleic acids, and tissue microarrays for genotyping or protein-level analyses by the MPP Core A. Inthis way, the MPP Core A will serve a critical validation function, relating the findings of the research projectsto human lung adenocarcinomas, and placing them in the context of key clinical, pathologic, mutational, andgenomic parameters. The MPP core will also provide 1) consistent, expert pathologic characterization of allhuman lung cancer specimens and related murine models, including tumor viability and assessment of tumorresponse to various investigational agents used in program projects; 2) construction of tissue microarrays forhigh throughput analysis of tissue samples 3) interpretation of immunohistochemical stains for validationstudies of various projects; and 4). optimization and evaluation of novel antibodies forimmunohistochemistry: The MPP Core A is an efficient, cost effective means to provide these necessaryresearch components to all program investigators.
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