Abstract

Electrophysiology and calcium imaging will be used to study olfactory receptor neuron (ORN) responsiveness in a novel tissue slice preparation.
Three specific aims are delineated. In the first specific aim, the sensitivity, selectivity and adaptive properties of the ORNs will be studied using loose, perforated and whole cell patch recording. In the second aim, imaging of intracellular calcium will be used to determine whether odors elicit stereotypic patterns of neuronal activity in the epithelium, and whether these patterns correlate with the olfactory receptor expression zones of the olfactory epithelium. Finally, in the third specific aim experiments are proposed to study responsiveness of ORN expressing a specific receptor (P2) in a gene-targeted mouse co-expressing the marker protein taulacZ.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Program Projects (P01)
Project #
5P01DC000347-16
Application #
6597578
Study Section
Communication Disorders Review Committee (CDRC)
Project Start
2002-04-01
Project End
2003-03-31
Budget Start
Budget End
Support Year
16
Fiscal Year
2002
Total Cost
$228,547
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Laaris, Nora; Puche, Adam; Ennis, Matthew (2007) Complementary postsynaptic activity patterns elicited in olfactory bulb by stimulation of mitral/tufted and centrifugal fiber inputs to granule cells. J Neurophysiol 97:296-306
Weiler, Elke (2005) Postnatal development of the rat vomeronasal organ. Chem Senses 30 Suppl 1:i127-8
Weiler, Elke; Farbman, Albert I (2003) The septal organ of the rat during postnatal development. Chem Senses 28:581-93
Suzuki, Y; Farbman, A I (2000) Tumor necrosis factor-alpha-induced apoptosis in olfactory epithelium in vitro: possible roles of caspase 1 (ICE), caspase 2 (ICH-1), and caspase 3 (CPP32). Exp Neurol 165:35-45
Farbman, A I; Ezeh, P I (2000) TGF-alpha and olfactory marker protein enhance mitosis in rat olfactory epithelium in vivo. Neuroreport 11:3655-8
Smith, D V; Som, J; Boughter Jr, J D et al. (1999) Cellular expression of alpha-gustducin and the A blood group antigen in rat fungiform taste buds cross-reinnervated by the IXth nerve. J Comp Neurol 409:118-30
Weiler, E; Apfelbach, R; Farbman, A I (1999) The vomeronasal organ of the male ferret. Chem Senses 24:127-36
Weiler, E; Farbman, A I (1999) Mitral cell loss following lateral olfactory tract transection increases proliferation density in rat olfactory epithelium. Eur J Neurosci 11:3265-75
Weiler, E; McCulloch, M A; Farbman, A I (1999) Proliferation in the vomeronasal organ of the rat during postnatal development. Eur J Neurosci 11:700-11
Carr, V M; Walters, E; Margolis, F L et al. (1998) An enhanced olfactory marker protein immunoreactivity in individual olfactory receptor neurons following olfactory bulbectomy may be related to increased neurogenesis. J Neurobiol 34:377-90

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