The propose of this research is to demonstrate and understand the development of individual thalamocortical fibers in their entirety from the ventroposterior medial nucleus of the thalamus to the somatosensory cortex of the rat and mouse. Individual neurons and their axons will be labeled by selective injection of various dyes in a slice preparation. We developed this preparation to capture the entire somatosensory thalamocortical pathway in a single tissue slice which may be kept in vitro for hours. Specifically a number of well filled axons will be harvested from normal animals, animals subjected to various peripheral and central lesions as neonates and ultimately in a series of developing animals. Animals will be prepared by the animal CORE. Our goal is to characterize these individual fibers from the viewpoint of competitive mechanisms that normally operate in the development of brain maps. Special attention will be given to comparisons with axons in other parts of the central trigeminal pathway as demonstrated in other projects in this grant and to take advantage of the expertise of the other investigators to add these specific data to complementary studies of axonal populations being carried out in our laboratory.

Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Saint Louis University
Department
Type
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
63103
Jacquin, Mark F; Arends, Joop J A; Renehan, William E et al. (2015) Whisker-related circuitry in the trigeminal nucleus principalis: Topographic precision. Somatosens Mot Res 32:8-20
Xiang, Chuanxi; Arends, Joop J A; Jacquin, Mark F (2014) Whisker-related circuitry in the trigeminal nucleus principalis: ultrastructure. Somatosens Mot Res 31:141-51
Vadivelu, Sudhakar; Platik, Marina M; Choi, Luke et al. (2005) Multi-germ layer lineage central nervous system repair: nerve and vascular cell generation by embryonic stem cells transplanted in the injured brain. J Neurosurg 103:124-35
Pluto, Charles P; Chiaia, Nicolas L; Rhoades, Robert W et al. (2005) Reducing contralateral SI activity reveals hindlimb receptive fields in the SI forelimb-stump representation of neonatally amputated rats. J Neurophysiol 94:1727-32
Genc, Baris; Ulupinar, Emel; Erzurumlu, Reha S (2005) Differential Trk expression in explant and dissociated trigeminal ganglion cell cultures. J Neurobiol 64:145-56
Gandhi, Rohan; Ryals, Janelle M; Wright, Douglas E (2004) Neurotrophin-3 reverses chronic mechanical hyperalgesia induced by intramuscular acid injection. J Neurosci 24:9405-13
McDonald, John W; Becker, Daniel; Holekamp, Terrence F et al. (2004) Repair of the injured spinal cord and the potential of embryonic stem cell transplantation. J Neurotrauma 21:383-93
Genc, Baris; Ozdinler, P Hande; Mendoza, April E et al. (2004) A chemoattractant role for NT-3 in proprioceptive axon guidance. PLoS Biol 2:e403
Ulupinar, Emel; Unal, Nedim; Erzurumlu, Reha S (2004) Morphometric analysis of embryonic rat trigeminal neurons treated with different neurotrophins. Anat Rec A Discov Mol Cell Evol Biol 277:396-407
Wright, Douglas E; Ryals, Janelle M; McCarson, Kenneth E et al. (2004) Diabetes-induced expression of activating transcription factor 3 in mouse primary sensory neurons. J Peripher Nerv Syst 9:242-54

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