Abstract

Tissue Culture Core will provide essential services and assistance to the investigators whose projects involve in vitro culture of mammalian cells. These services will include the propagation of stock cell lines (Caco-2, COS-1, HepG-2, T84, IEC-6, 3T3, and AR 427) and special cell lines (fibroblasts from normals and patients with LCAD deficiency) initiated by the investigators. Cells will be maintained for individual investigators and prepared for cryo-preservation and storage for future applications. Cells grown in flasks or on filters will be provided to investigators for the performance of experiments. The reliable production od reproducible cell cultures is time consuming and exacting. Prevention of contamination requires the presence of an isolatable tissue culture facility and limited access of personnel to that facility. In addition, several cell lines (Caco-2, COS-1, HepG-2) will be used by more than one of the investigators in the program project. Culturing of these cell lines in a central facility enhances the efficiency of tissue culture and also provides uniform cells for use in the different projects. The uniformity of the cells used in the different projects allows for easier collaboration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK033487-14
Application #
6301094
Study Section
Project Start
2000-01-01
Project End
2001-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
2000
Total Cost
$155,266
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Sukumar, N; Mathews, F S; Gordon, M M et al. (2009) Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals. Cryst Growth Des 9:348-351
Mathews, F S; Gordon, M M; Chen, Z et al. (2007) Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution. Proc Natl Acad Sci U S A 104:17311-6
Mahmood, Akhtar; Shao, Jian-su; Alpers, David H (2003) Rat enterocytes secrete SLPs containing alkaline phosphatase and cubilin in response to corn oil feeding. Am J Physiol Gastrointest Liver Physiol 285:G433-41
Shao, J; Sartor, R B; Dial, E et al. (2000) Expression of intrinsic factor in rat and murine gastric mucosal cell lineages is modified by inflammation. Am J Pathol 157:1197-205
Wen, J; Kinnear, M B; Richardson, M A et al. (2000) Functional expression in Pichia pastoris of human and rat intrinsic factor. Biochim Biophys Acta 1490:43-53
Brada, N; Gordon, M M; Shao, J S et al. (2000) Production of gastric intrinsic factor, transcobalamin, and haptocorrin in opossum kidney cells. Am J Physiol Renal Physiol 279:F1006-13
Zhou, Y; Kelly, D P; Strauss, A W et al. (1999) Characterization of the human very-long-chain acyl-CoA dehydrogenase gene promoter region: a role for activator protein 2. Mol Genet Metab 68:481-7
Syder, A J; Guruge, J L; Li, Q et al. (1999) Helicobacter pylori attaches to NeuAc alpha 2,3Gal beta 1,4 glycoconjugates produced in the stomach of transgenic mice lacking parietal cells. Mol Cell 3:263-74
Mathur, A; Sims, H F; Gopalakrishnan, D et al. (1999) Molecular heterogeneity in very-long-chain acyl-CoA dehydrogenase deficiency causing pediatric cardiomyopathy and sudden death. Circulation 99:1337-43
Li, Q; Karam, S M; Coerver, K A et al. (1998) Stimulation of activin receptor II signaling pathways inhibits differentiation of multiple gastric epithelial lineages. Mol Endocrinol 12:181-92

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