Due to the emphasis of molecular biological approaches in the program project, we intend to establish a molecular biology core facility for use by research projects associated with the program project. One mission of the core facility will be to synthesize oligonucleotides to be used by various projects for PCR, site-directed mutagenesis, and DNA sequencing applications. In addition, core personnel will use site-directed mutagenesis to construct a library of CFTR CDNA constructs containing published, naturally occurring mutations. This library will be updated periodically and these constructs will be available to investigators to facilitate the analysis of mutations using the expression systems and analytical methods described in individual proposals. This will eliminate the redundancy that would result if each group prepared these constructs separately, and will also allow us to maintain greater quality control during the mutagenesis protocol. The facility will also carry out PCR applications, DNA sequencing, and Northern and Southern blots for investigators as needed.

Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Schultz, B D; Frizzell, R A; Bridges, R J (1999) Rescue of dysfunctional deltaF508-CFTR chloride channel activity by IBMX. J Membr Biol 170:51-66
Dubinsky, W P; Mayorga-Wark, O; Schultz, S G (1998) Colocalization of glycolytic enzyme activity and KATP channels in basolateral membrane of Necturus enterocytes. Am J Physiol 275:C1653-9
Schultz, B D; DeRoos, A D; Venglarik, C J et al. (1996) Glibenclamide blockade of CFTR chloride channels. Am J Physiol 271:L192-200
Mayorga-Wark, O; Dubinsky, W P; Schultz, S G (1996) Reversal of glibenclamide and voltage block of an epithelial KATP channel. Am J Physiol 271:C1122-30
Dong, J Y; Wang, D; Van Ginkel, F W et al. (1996) Systematic analysis of repeated gene delivery into animal lungs with a recombinant adenovirus vector. Hum Gene Ther 7:319-31
Schultz, B D; Bridges, R J; Frizzell, R A (1996) Lack of conventional ATPase properties in CFTR chloride channel gating. J Membr Biol 151:63-75
Howard, M; DuVall, M D; Devor, D C et al. (1995) Epitope tagging permits cell surface detection of functional CFTR. Am J Physiol 269:C1565-76
Frizzell, R A (1995) Functions of the cystic fibrosis transmembrane conductance regulator protein. Am J Respir Crit Care Med 151:S54-8
Mayorga-Wark, O; Dubinsky, W P; Schultz, S G (1995) Reconstitution of a KATP channel from basolateral membranes of Necturus enterocytes. Am J Physiol 269:C464-71
Schultz, B D; Venglarik, C J; Bridges, R J et al. (1995) Regulation of CFTR Cl- channel gating by ADP and ATP analogues. J Gen Physiol 105:329-61

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